Mapping the kinetochore MAP functions required for stabilizing microtubule attachments to chromosomes during metaphase

Mohammed A. Amin, Shivangi Agarwal, Dileep Varma*

*Corresponding author for this work

Research output: Contribution to journalReview article

1 Scopus citations

Abstract

In mitosis, faithful chromosome segregation is orchestrated by the dynamic interactions between the spindle microtubules (MTs) emanating from the opposite poles and the kinetochores of the chromosomes. However, the precise mechanism that coordinates the coupling of the kinetochore components to dynamic MTs has been a long-standing question. Microtubule-associated proteins (MAPs) regulate MT nucleation and dynamics, MT-mediated transport and MT cross-linking in cells. During mitosis, MAPs play an essential role not only in determining spindle length, position, and orientation but also in facilitating robust kinetochore-microtubule (kMT) attachments by linking the kinetochores to spindle MTs efficiently. The stability of MTs imparted by the MAPs is critical to ensure accurate chromosome segregation. This review primarily focuses on the specific function of nonmotor kinetochore MAPs, their recruitment to kinetochores and their MT-binding properties. We also attempt to synthesize and strengthen our understanding of how these MAPs work in coordination with the kinetochore-bound Ndc80 complex (the key component at the MT-binding interface in metaphase and anaphase) to establish stable kMT attachments and control accurate chromosome segregation during mitosis.

Original languageEnglish (US)
Pages (from-to)398-412
Number of pages15
JournalCytoskeleton
Volume76
Issue number6
DOIs
StatePublished - Jun 1 2019

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Keywords

  • MAPs
  • Ndc80
  • chromosomes
  • kinetochores
  • microtubules
  • mitosis

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

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