Mapping the subcellular localization of Fe3O 4@TiO2 nanoparticles by X-ray Fluorescence Microscopy

Y. Yuan, S. Chen, S. C. Gleber, B. Lai, K. Brister, C. Flachenecker, B. Wanzer, T. Paunesku, S. Vogt, G. E. Woloschak

Research output: Contribution to journalConference article

1 Citation (Scopus)

Abstract

The targeted delivery of Fe3O4@TiO2 nanoparticles to cancer cells is an important step in their development as nanomedicines. We have synthesized nanoparticles that can bind the Epidermal Growth Factor Receptor, a cell surface protein that is overexpressed in many epithelial type cancers. In order to study the subcellular distribution of these nanoparticles, we have utilized the sub-micron resolution of X-ray Fluorescence Microscopy to map the location of Fe3O4@TiO2 NPs and other trace metal elements within HeLa cervical cancer cells. Here we demonstrate how the higher resolution of the newly installed Bionanoprobe at the Advanced Photon Source at Argonne National Laboratory can greatly improve our ability to distinguish intracellular nanoparticles and their spatial relationship with subcellular compartments.

Original languageEnglish (US)
Article number012020
JournalJournal of Physics: Conference Series
Volume463
Issue number1
DOIs
StatePublished - Jan 1 2013
Event11th International Conference on X-Ray Microscopy, XRM 2012 - Shanghai, China
Duration: Aug 5 2012Aug 10 2012

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microscopy
fluorescence
nanoparticles
cancer
x rays
compartments
delivery
proteins
high resolution
photons
cells
metals

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Yuan, Y. ; Chen, S. ; Gleber, S. C. ; Lai, B. ; Brister, K. ; Flachenecker, C. ; Wanzer, B. ; Paunesku, T. ; Vogt, S. ; Woloschak, G. E. / Mapping the subcellular localization of Fe3O 4@TiO2 nanoparticles by X-ray Fluorescence Microscopy. In: Journal of Physics: Conference Series. 2013 ; Vol. 463, No. 1.
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abstract = "The targeted delivery of Fe3O4@TiO2 nanoparticles to cancer cells is an important step in their development as nanomedicines. We have synthesized nanoparticles that can bind the Epidermal Growth Factor Receptor, a cell surface protein that is overexpressed in many epithelial type cancers. In order to study the subcellular distribution of these nanoparticles, we have utilized the sub-micron resolution of X-ray Fluorescence Microscopy to map the location of Fe3O4@TiO2 NPs and other trace metal elements within HeLa cervical cancer cells. Here we demonstrate how the higher resolution of the newly installed Bionanoprobe at the Advanced Photon Source at Argonne National Laboratory can greatly improve our ability to distinguish intracellular nanoparticles and their spatial relationship with subcellular compartments.",
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Mapping the subcellular localization of Fe3O 4@TiO2 nanoparticles by X-ray Fluorescence Microscopy. / Yuan, Y.; Chen, S.; Gleber, S. C.; Lai, B.; Brister, K.; Flachenecker, C.; Wanzer, B.; Paunesku, T.; Vogt, S.; Woloschak, G. E.

In: Journal of Physics: Conference Series, Vol. 463, No. 1, 012020, 01.01.2013.

Research output: Contribution to journalConference article

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T1 - Mapping the subcellular localization of Fe3O 4@TiO2 nanoparticles by X-ray Fluorescence Microscopy

AU - Yuan, Y.

AU - Chen, S.

AU - Gleber, S. C.

AU - Lai, B.

AU - Brister, K.

AU - Flachenecker, C.

AU - Wanzer, B.

AU - Paunesku, T.

AU - Vogt, S.

AU - Woloschak, G. E.

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AB - The targeted delivery of Fe3O4@TiO2 nanoparticles to cancer cells is an important step in their development as nanomedicines. We have synthesized nanoparticles that can bind the Epidermal Growth Factor Receptor, a cell surface protein that is overexpressed in many epithelial type cancers. In order to study the subcellular distribution of these nanoparticles, we have utilized the sub-micron resolution of X-ray Fluorescence Microscopy to map the location of Fe3O4@TiO2 NPs and other trace metal elements within HeLa cervical cancer cells. Here we demonstrate how the higher resolution of the newly installed Bionanoprobe at the Advanced Photon Source at Argonne National Laboratory can greatly improve our ability to distinguish intracellular nanoparticles and their spatial relationship with subcellular compartments.

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