Marker rescue of a transformation-negative Epstein-Barr virus recombinant from an infected Burkitt lymphoma cell line: A method useful for analysis of genes essential for transformation

Andrew Marchini, Elliott Kieff, Richard Longnecker*

*Corresponding author for this work

Research output: Contribution to journalComment/debatepeer-review

13 Scopus citations

Abstract

A Burkitt lymphoma cell line infected in vitro with a transformation- defective mutant recombinant Epstein-Barr virus (EBV) was used to attempt marker rescue of transformation competence by transfection with cloned wild- type DNA. EBV replication was induced in the transfected cells, and wild- type EBV DNA recombined via flanking homologous sequences adjacent to the deletion, resulting in a virus which transformed primary B lymphocytes in vitro. This strategy should be useful for molecular genetic analysis of the role of part or all of any gene in cell growth transformation.

Original languageEnglish (US)
Pages (from-to)606-609
Number of pages4
JournalJournal of virology
Volume67
Issue number1
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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