Abstract
Follicular lymphoma (FL) is an indolent B cell malignancy characterized by an extensive but poorly functional T cell infiltrate in the tumor microenvironment. Using mass cytometry, we identified at least 12 subsets of intratumoral CD4 + T cells, 3 of which were unique to FL biopsies versus control tissues. Of these subsets, the frequency of naive T cells correlated with improved patient survival. Although total PD-1 + T cell numbers were not associated with patient outcome, specific PD-1 + T cell subpopulations were associated with poor survival. Intratumoral T cells lacking CD27 and CD28 co-stimulatory receptor expression were enriched in FL and correlated with inferior patient outcomes. In vitro models revealed that CD70 + lymphoma cells played an important role in expanding this population. Taken together, our mass cytometry results identified CD4 + memory T cell populations that are poorly functional due to loss of co-stimulatory receptor expression and are associated with an inferior survival in FL. Yang et al. utilize mass cytometry (CyTOF) to characterize intratumoral T cells and explore the clinical relevance of T cell subsets in follicular lymphoma (FL). Clustering analysis reveals an immune signature with reduced expression of co-stimulatory molecules on intratumoral T cells that correlated with a poor prognosis in FL.
Original language | English (US) |
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Pages (from-to) | 2178-2193.e3 |
Journal | Cell reports |
Volume | 26 |
Issue number | 8 |
DOIs | |
State | Published - Feb 19 2019 |
Externally published | Yes |
Keywords
- CD27
- CD4 CD25 regulatory T cells
- CyTOF
- PD-1
- co-stimulatory receptor
- follicular lymphoma
- immune signature
- intratumoral CD4 T cell
- mass cytometry
- patient survival
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)