Mast cell-deficient mice develop a reaginic antibody immune response

M. R. Saban, D. DeBoer, B. Wershil, M. Zine, D. Bjorling, R. Saban

Research output: Contribution to journalArticlepeer-review

Abstract

In using a mast cell-deficient mouse model to investigate the role of mast cells and immediate-type hypersensitivity in antigen-induced bladder inflammation, we initially sought to demonstrate that mast cell-deficient mice can mount a reaginic antibody response. Mast cell-deficient (w/wv) mice and congenic controls (+/+) were actively sensitized by repeated injections of dinitrophenol-ovalbumin (DNP-OVA). Serum IgE was measured by ELISA before and after DNP-OVA. After 4 weeks, the urinary bladders were removed and suspended in a tissue bath. Smooth muscle contraction and histamine release were monitored upon challenge of bladder tissue by superfusion with DNP-HSA. In similar experiments, serum from sensitized mice was used for passive sensitization of bladder tissue from naive mice. Pre-sensitization serum IgE was similar in w/wv and +/+ mice (0.4 vs. 0.3 μg/ml); IgE levels increased significantly in both groups after active sensitization (to 2.9 vs. 2.0 μg/ml). Bladders from actively-sensitized w/wv mice failed to respond to antigen challenge, whereas those from actively-sensitized +/+ mice responded with contraction and histamine release. Bladders isolated from naive +/+ mice, when passively sensitized with serum from sensitized w/wv mice, also contracted upon antigen stimulation. These studies demonstrate that w/wv mast cell deficient mice produce functional reaginic antibodies; the experimental approach described can provide quantitative data and will aid in defining the role of mast cells in bladder inflammatory reactions.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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