Mast cell regulation of inflammation and gene expression during antigen-induced bladder inflammation in mice.

R. Saban*, M. R. Saban, N. B. Nguyen, T. G. Hammond, B. K. Wershil

*Corresponding author for this work

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Mast cell numbers are significantly increased in bladder disorders including malignancy and interstitial cystitis, but their precise role has been difficult to determine. We characterized the role of mast cells on gene regulation associated with antigen-induced bladder inflammation in mice. For this purpose, we examined the responses in mast cell-deficient (Kit(W)/Kit(W-v)), congenic normal (+/+), and Kit(W)/Kit(W-v) mice that were reconstituted with bone marrow stem cells (BMR) to restore mast cells. All mice were actively sensitized and challenged intravesically with either saline or specific antigen. Bladder inflammation occurred in +/+ and BMR but not the Kit(W)/Kit(W-v) mice. Gene expression was determined using mouse cDNA expression arrays. Self-organizing maps, performed without preconditions, indicated gene expression changes dependent on the presence of mast cells. These genes were upregulated in bladders isolated from antigen challenge of +/+, not altered in Kit(W)/Kit(W-v), and were upregulated in BMR mice. Taken together these results demonstrate an important role for mast cells in allergic cystitis and indicate that mast cells can alter their environment by regulating tissue gene expression.

Original languageEnglish (US)
Pages (from-to)35-43
Number of pages9
JournalPhysiological Genomics
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2001

Keywords

  • Gene array
  • Inflammation
  • Interstitial cystitis
  • Mast cell-deficient mice
  • Mast cells
  • Self-organizing maps

ASJC Scopus subject areas

  • Physiology
  • Genetics

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