Mast cells contribute to PACAP-induced dermal oedema in mice

Anjona Schmidt-Choudhury; Glenn T. Furuta; Stephen J. Galli; Wolfgang E. Schmidt; Barry K. Wershil

doi:10.1016/S0167-0115(99)00041-5

Mast cells contribute to PACAP-induced dermal oedema in mice

Anjona Schmidt-Choudhury^*, Glenn T. Furuta, Stephen J. Galli, Wolfgang E. Schmidt, Barry K. Wershil

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

In the present study the effect of intradermal PACAP-injection on dermal oedema in mice was investigated and the contribution of mast cells to this response was assessed. The injection of PACAP 1-38 into the ears of C57BL/6 mice evoked a dose-dependent response, which, after higher doses of PACAP 1-38, lasted at least 24 h. Histological examination showed significant mast cell degranulation induced by PACAP. Using mast cell-deficient WBB6F₁-Kit(W)/Kit(W-v) mice and the congenic mice, we demonstrated that the the early phase (30 min to 6 h) of PACAP-induced ear swelling response was significantly diminished in mast cell-deficient mice, suggesting that mast cell degranulation contributes to this phase of the response. When mast cell-deficient WBB6F₁-Kit(W)/Kit(W-v) mice were locally and selectively reconstituted by adoptive mast cell transfer, the dermal oedema was almost equal to that of control animals in the early phase of PACAP injection. These results show that mast cell degranulation contributes to PACAP-induced dermal oedema in mice. Copyright (C) 1999 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	65-69
Number of pages	5
Journal	Regulatory Peptides
Volume	82
Issue number	1-3
DOIs	https://doi.org/10.1016/S0167-0115(99)00041-5
State	Published - Jun 30 1999

Funding

We thank Jackie A. Lavigne for expert technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft, DFG, Ro 819-1/1, Schm 1223/4-1 (A.S.-C.) and Schm 805/4-2 (W.E.S.), NIH grants DK33506 and DK46819 (B.K.W.) and CA72074 and AI23990 (S.J.G.).

Keywords

Ear swelling
Mast cell-deficient mice
PACAP 1-38

ASJC Scopus subject areas

Endocrinology
Cellular and Molecular Neuroscience
Biochemistry
Physiology
Clinical Biochemistry

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10.1016/S0167-0115(99)00041-5

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title = "Mast cells contribute to PACAP-induced dermal oedema in mice",

abstract = "In the present study the effect of intradermal PACAP-injection on dermal oedema in mice was investigated and the contribution of mast cells to this response was assessed. The injection of PACAP 1-38 into the ears of C57BL/6 mice evoked a dose-dependent response, which, after higher doses of PACAP 1-38, lasted at least 24 h. Histological examination showed significant mast cell degranulation induced by PACAP. Using mast cell-deficient WBB6F1-Kit(W)/Kit(W-v) mice and the congenic mice, we demonstrated that the the early phase (30 min to 6 h) of PACAP-induced ear swelling response was significantly diminished in mast cell-deficient mice, suggesting that mast cell degranulation contributes to this phase of the response. When mast cell-deficient WBB6F1-Kit(W)/Kit(W-v) mice were locally and selectively reconstituted by adoptive mast cell transfer, the dermal oedema was almost equal to that of control animals in the early phase of PACAP injection. These results show that mast cell degranulation contributes to PACAP-induced dermal oedema in mice. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Ear swelling, Mast cell-deficient mice, PACAP 1-38",

author = "Anjona Schmidt-Choudhury and Furuta, {Glenn T.} and Galli, {Stephen J.} and Schmidt, {Wolfgang E.} and Wershil, {Barry K.}",

note = "Funding Information: We thank Jackie A. Lavigne for expert technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft, DFG, Ro 819-1/1, Schm 1223/4-1 (A.S.-C.) and Schm 805/4-2 (W.E.S.), NIH grants DK33506 and DK46819 (B.K.W.) and CA72074 and AI23990 (S.J.G.). ",

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AU - Wershil, Barry K.

N1 - Funding Information: We thank Jackie A. Lavigne for expert technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft, DFG, Ro 819-1/1, Schm 1223/4-1 (A.S.-C.) and Schm 805/4-2 (W.E.S.), NIH grants DK33506 and DK46819 (B.K.W.) and CA72074 and AI23990 (S.J.G.).

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N2 - In the present study the effect of intradermal PACAP-injection on dermal oedema in mice was investigated and the contribution of mast cells to this response was assessed. The injection of PACAP 1-38 into the ears of C57BL/6 mice evoked a dose-dependent response, which, after higher doses of PACAP 1-38, lasted at least 24 h. Histological examination showed significant mast cell degranulation induced by PACAP. Using mast cell-deficient WBB6F1-Kit(W)/Kit(W-v) mice and the congenic mice, we demonstrated that the the early phase (30 min to 6 h) of PACAP-induced ear swelling response was significantly diminished in mast cell-deficient mice, suggesting that mast cell degranulation contributes to this phase of the response. When mast cell-deficient WBB6F1-Kit(W)/Kit(W-v) mice were locally and selectively reconstituted by adoptive mast cell transfer, the dermal oedema was almost equal to that of control animals in the early phase of PACAP injection. These results show that mast cell degranulation contributes to PACAP-induced dermal oedema in mice. Copyright (C) 1999 Elsevier Science B.V.

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Mast cells contribute to PACAP-induced dermal oedema in mice

Abstract

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Keywords

ASJC Scopus subject areas

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