Mast cells contribute to PACAP-induced dermal oedema in mice

Anjona Schmidt-Choudhury*, Glenn T. Furuta, Stephen J. Galli, Wolfgang E. Schmidt, Barry K. Wershil

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


In the present study the effect of intradermal PACAP-injection on dermal oedema in mice was investigated and the contribution of mast cells to this response was assessed. The injection of PACAP 1-38 into the ears of C57BL/6 mice evoked a dose-dependent response, which, after higher doses of PACAP 1-38, lasted at least 24 h. Histological examination showed significant mast cell degranulation induced by PACAP. Using mast cell-deficient WBB6F1-Kit(W)/Kit(W-v) mice and the congenic mice, we demonstrated that the the early phase (30 min to 6 h) of PACAP-induced ear swelling response was significantly diminished in mast cell-deficient mice, suggesting that mast cell degranulation contributes to this phase of the response. When mast cell-deficient WBB6F1-Kit(W)/Kit(W-v) mice were locally and selectively reconstituted by adoptive mast cell transfer, the dermal oedema was almost equal to that of control animals in the early phase of PACAP injection. These results show that mast cell degranulation contributes to PACAP-induced dermal oedema in mice. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalRegulatory Peptides
Issue number1-3
StatePublished - Jun 30 1999


  • Ear swelling
  • Mast cell-deficient mice
  • PACAP 1-38

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience


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