Mast cells mediate substance P-induced bladder inflammation through an NK1 receptor-independent mechanism

Ricardo Saban*, Norma P. Gerard, Marcia R. Saban, Ngoc Bich Nguyen, Douglas J. Deboer, Barry K. Wershil

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


The role of neurokinin-1 receptors (NK1R) in the interaction between mast cells and substance P (SP) in bladder inflammation was determined. Mast cell-deficient KitW/KitW-v, congenic normal (+/+), and KitW/KitW-v mice that were reconstituted with bone marrow cells isolated from NK1R-/- mice were challenged by instillation of SP, antigen, or saline into the urinary bladder. Twenty-four hours after challenge, the bladders were prepared for morphological assessment and gene expression. SP-induced bladder inflammation was mast cell dependent and did not require NK1R expression on the mast cell. Cluster analysis identified functionally significant genes that were dependent on the presence of mast cells for their upregulation regardless of stimulus. Those include serine protein inhibitor 2.2, maspin, mitogen- and stress-activated protein kinase 2, and macrophage colony-stimulating factor 1. Our findings demonstrate that while mast cells are essential for both antigen- and SP-induced bladder inflammation, there are common genes and unique genes expressed in each type of inflammatory reaction. When combined with unique animal models, gene array analysis provides a useful approach for identifying and characterizing pathways involved in bladder inflammation.

Original languageEnglish (US)
Pages (from-to)F616-F629
JournalAmerican Journal of Physiology - Renal Physiology
Issue number4 52-4
StatePublished - Oct 2002


  • Gene regulation
  • Inflammation
  • Mast cells
  • Protein kinases/phosphatases
  • Transgenic/knockout

ASJC Scopus subject areas

  • Physiology
  • Urology


Dive into the research topics of 'Mast cells mediate substance P-induced bladder inflammation through an NK1 receptor-independent mechanism'. Together they form a unique fingerprint.

Cite this