TY - JOUR
T1 - Matching nucleotide sequences of human antibodies with other known sequences
AU - Wu, Tai Te
N1 - Funding Information:
The research was supported by a grant from the Leukemia Research Foundation of Chicago. Work with the PROPHET computer system was supported by National Institutes of Health Contract NO12-RR-8-2118.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1988/3/21
Y1 - 1988/3/21
N2 - From an evolutionary point of view, the complementarity-determining regions of antibodies are distinct from other proteins including the framework regions of antibodies. A search for identical nucleotide sequences of eighty-four 15 consecutive bp in the complementarity-determining regions of human antibody heavy chains with other known sequences yielded four matches: two sequential 15-bp matches, or one 16-bp match, with the coding region of a sea-urchin testis histone H2b-2, one 15-bp match with the promotor region of a cauliflower mosaic virus inclusion body protein, and a 15-bp match with an intron between exons 1 and 2 of human factor IX. As a control, an identical search of eighty-four 15 consecutive bp in the framework regions of human antibody heavy chains yielded no matches with other sequences except those from other antibody framwork regions. Since the currently available nucleotide sequence database used in the search consisted of about 1 × 107 bp, finding such matches in the complementarity-determining regions might not be random.
AB - From an evolutionary point of view, the complementarity-determining regions of antibodies are distinct from other proteins including the framework regions of antibodies. A search for identical nucleotide sequences of eighty-four 15 consecutive bp in the complementarity-determining regions of human antibody heavy chains with other known sequences yielded four matches: two sequential 15-bp matches, or one 16-bp match, with the coding region of a sea-urchin testis histone H2b-2, one 15-bp match with the promotor region of a cauliflower mosaic virus inclusion body protein, and a 15-bp match with an intron between exons 1 and 2 of human factor IX. As a control, an identical search of eighty-four 15 consecutive bp in the framework regions of human antibody heavy chains yielded no matches with other sequences except those from other antibody framwork regions. Since the currently available nucleotide sequence database used in the search consisted of about 1 × 107 bp, finding such matches in the complementarity-determining regions might not be random.
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U2 - 10.1016/S0022-5193(88)80239-X
DO - 10.1016/S0022-5193(88)80239-X
M3 - Article
C2 - 3136277
AN - SCOPUS:0023907723
SN - 0022-5193
VL - 131
SP - 231
EP - 234
JO - Journal of Theoretical Biology
JF - Journal of Theoretical Biology
IS - 2
ER -