Maturation and death in terminal megakaryocytic differentiation: Phenotypic and DNA-microarray characterization

Peter G. Fuhrken; Chi Chen; Lisa M. Giammona; William M. Miller; Eleftherios T. Papoutsakis

Maturation and death in terminal megakaryocytic differentiation: Phenotypic and DNA-microarray characterization

Peter G. Fuhrken^*, Chi Chen, Lisa M. Giammona, William M. Miller, Eleftherios T. Papoutsakis

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

The ex vivo culture of hematopoietic stem cells (HJSC) was proposed to produce transplantable megakaryocytes. The cell line was induced to undergo terminal differentiation upon continuous treatment with the protein kinase C-stimulator phorbol 12-myristate 13-acetate (PMA). The transcriptional state of the CHRF cell line to the corresponding points in primary cell cultures was mapped to define the position of common model cell line in the pathway of megakaryocytic differentiation. The results show that the independent transcriptional data set for cultured megakaryocytes derived from mobilized peripheral blood Cd34+ primary cells.

Original language	English (US)
Pages	8731
Number of pages	1
State	Published - 2004
Event	2004 AIChE Annual Meeting - Austin, TX, United States Duration: Nov 7 2004 → Nov 12 2004

Other

Other	2004 AIChE Annual Meeting
Country/Territory	United States
City	Austin, TX
Period	11/7/04 → 11/12/04

ASJC Scopus subject areas

General Engineering

Cite this

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title = "Maturation and death in terminal megakaryocytic differentiation: Phenotypic and DNA-microarray characterization",

abstract = "The ex vivo culture of hematopoietic stem cells (HJSC) was proposed to produce transplantable megakaryocytes. The cell line was induced to undergo terminal differentiation upon continuous treatment with the protein kinase C-stimulator phorbol 12-myristate 13-acetate (PMA). The transcriptional state of the CHRF cell line to the corresponding points in primary cell cultures was mapped to define the position of common model cell line in the pathway of megakaryocytic differentiation. The results show that the independent transcriptional data set for cultured megakaryocytes derived from mobilized peripheral blood Cd34+ primary cells.",

author = "Fuhrken, {Peter G.} and Chi Chen and Giammona, {Lisa M.} and Miller, {William M.} and Papoutsakis, {Eleftherios T.}",

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AU - Fuhrken, Peter G.

AU - Chen, Chi

AU - Giammona, Lisa M.

AU - Miller, William M.

AU - Papoutsakis, Eleftherios T.

PY - 2004

Y1 - 2004

N2 - The ex vivo culture of hematopoietic stem cells (HJSC) was proposed to produce transplantable megakaryocytes. The cell line was induced to undergo terminal differentiation upon continuous treatment with the protein kinase C-stimulator phorbol 12-myristate 13-acetate (PMA). The transcriptional state of the CHRF cell line to the corresponding points in primary cell cultures was mapped to define the position of common model cell line in the pathway of megakaryocytic differentiation. The results show that the independent transcriptional data set for cultured megakaryocytes derived from mobilized peripheral blood Cd34+ primary cells.

AB - The ex vivo culture of hematopoietic stem cells (HJSC) was proposed to produce transplantable megakaryocytes. The cell line was induced to undergo terminal differentiation upon continuous treatment with the protein kinase C-stimulator phorbol 12-myristate 13-acetate (PMA). The transcriptional state of the CHRF cell line to the corresponding points in primary cell cultures was mapped to define the position of common model cell line in the pathway of megakaryocytic differentiation. The results show that the independent transcriptional data set for cultured megakaryocytes derived from mobilized peripheral blood Cd34+ primary cells.

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Y2 - 7 November 2004 through 12 November 2004

ER -

Maturation and death in terminal megakaryocytic differentiation: Phenotypic and DNA-microarray characterization

Abstract

Other

ASJC Scopus subject areas

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