Maturation and death in terminal megakaryocytic differentiation: Phenotypic and DNA-microarray characterization

Peter G. Fuhrken*, Chi Chen, Lisa M. Giammona, William M Miller, Eleftherios T. Papoutsakis

*Corresponding author for this work

Research output: Contribution to conferencePaper

Abstract

The ex vivo culture of hematopoietic stem cells (HJSC) was proposed to produce transplantable megakaryocytes. The cell line was induced to undergo terminal differentiation upon continuous treatment with the protein kinase C-stimulator phorbol 12-myristate 13-acetate (PMA). The transcriptional state of the CHRF cell line to the corresponding points in primary cell cultures was mapped to define the position of common model cell line in the pathway of megakaryocytic differentiation. The results show that the independent transcriptional data set for cultured megakaryocytes derived from mobilized peripheral blood Cd34+ primary cells.

Original languageEnglish (US)
Number of pages1
StatePublished - Dec 1 2004
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

ASJC Scopus subject areas

  • Engineering(all)

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    Fuhrken, P. G., Chen, C., Giammona, L. M., Miller, W. M., & Papoutsakis, E. T. (2004). Maturation and death in terminal megakaryocytic differentiation: Phenotypic and DNA-microarray characterization. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.