TY - JOUR
T1 - Maximizing DNA loading on a range of gold nanoparticle sizes
AU - Hurst, Sarah J.
AU - Lytton-Jean, Abigail K.R.
AU - Mirkin, Chad A.
PY - 2006/12/15
Y1 - 2006/12/15
N2 - We have investigated the variables that influence DNA coverage on gold nanoparticles. The effects of salt concentration, spacer composition, nanoparticle size, and degree of sonication have been evaluated. Maximum loading was obtained by salt aging the nanoparticles to ∼0.7 M NaCl in the presence of DNA containing a poly(ethylene glycol) spacer. In addition, DNA loading was substantially increased by sonicating the nanoparticles during the surface loading process. Last, nanoparticles up to 250 nm in diameter were found have ∼2 orders of magnitude higher DNA loading than smaller (13-30 nm) nanoparticles, a consequence of their larger surface area. Stable large particles are attractive for a variety of biodiagnostic assays.
AB - We have investigated the variables that influence DNA coverage on gold nanoparticles. The effects of salt concentration, spacer composition, nanoparticle size, and degree of sonication have been evaluated. Maximum loading was obtained by salt aging the nanoparticles to ∼0.7 M NaCl in the presence of DNA containing a poly(ethylene glycol) spacer. In addition, DNA loading was substantially increased by sonicating the nanoparticles during the surface loading process. Last, nanoparticles up to 250 nm in diameter were found have ∼2 orders of magnitude higher DNA loading than smaller (13-30 nm) nanoparticles, a consequence of their larger surface area. Stable large particles are attractive for a variety of biodiagnostic assays.
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U2 - 10.1021/ac0613582
DO - 10.1021/ac0613582
M3 - Article
C2 - 17165821
AN - SCOPUS:33845573598
SN - 0003-2700
VL - 78
SP - 8313
EP - 8318
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 24
ER -