MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein

Mario Clemente Estable; Mojgan H. Naghavi; Hiroyuki Kato; Hua Xiao; Jun Qin; Anders Vahlne; Robert G. Roeder

doi:10.1159/000059424

MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein

Mario Clemente Estable, Mojgan H. Naghavi, Hiroyuki Kato, Hua Xiao, Jun Qin, Anders Vahlne, Robert G. Roeder^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Positive transcription elongation factor-b (P-TEFb) contains CDK9 and cyclin T₁. P-TEFb was affinity purified from a stably transfected cell line that expresses epitope-tagged CDK9, and proteins that appeared to be specifically bound were sequenced. In addition to CDK9, previously identified isoforms of cyclin T (including T₁, T_2A and T_2B), HSP90 and CDC37, this analysis identified a novel protein named MCEF. Cloning of its cognate cDNA revealed that MCEF is the newest member of the AF4 family of transcription factors involved in acute lymphoblastic leukemia. MCEF RNA was expressed in all human tissues examined, and antisera directed against recombinant MCEF specifically immunoprecipitated P-TEFb. Ectopic expression of MCEF did not activate HIV-1 replication, and tethering of MCEF to a promoter did not activate transcription.

Original language	English (US)
Pages (from-to)	234-245
Number of pages	12
Journal	Journal of Biomedical Science
Volume	9
Issue number	3
DOIs	https://doi.org/10.1159/000059424
State	Published - 2002

Keywords

CDK9
HIV
Leukemia
Positive transcription elongation factor-b
Transcription

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Molecular Biology
Clinical Biochemistry
Cell Biology
Biochemistry, medical
Pharmacology (medical)

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title = "MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein",

abstract = "Positive transcription elongation factor-b (P-TEFb) contains CDK9 and cyclin T1. P-TEFb was affinity purified from a stably transfected cell line that expresses epitope-tagged CDK9, and proteins that appeared to be specifically bound were sequenced. In addition to CDK9, previously identified isoforms of cyclin T (including T1, T2A and T2B), HSP90 and CDC37, this analysis identified a novel protein named MCEF. Cloning of its cognate cDNA revealed that MCEF is the newest member of the AF4 family of transcription factors involved in acute lymphoblastic leukemia. MCEF RNA was expressed in all human tissues examined, and antisera directed against recombinant MCEF specifically immunoprecipitated P-TEFb. Ectopic expression of MCEF did not activate HIV-1 replication, and tethering of MCEF to a promoter did not activate transcription.",

keywords = "CDK9, HIV, Leukemia, Positive transcription elongation factor-b, Transcription",

author = "Estable, {Mario Clemente} and Naghavi, {Mojgan H.} and Hiroyuki Kato and Hua Xiao and Jun Qin and Anders Vahlne and Roeder, {Robert G.}",

note = "Funding Information: The authors thank M. Guermah, S. Malik and V. Palhan fbr advice throughout this work and S.M., M.G. and M. Teichmann tbr careful reading of the manuscript. M.C.E. is a Fellow of the Canadian Institutes of Health Research (formerly MRC). {"}['his work was supported by NIH grant AI37327-05 to R.G.R.",

year = "2002",

doi = "10.1159/000059424",

language = "English (US)",

volume = "9",

pages = "234--245",

journal = "Journal of Biomedical Science",

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MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein. / Estable, Mario Clemente; Naghavi, Mojgan H.; Kato, Hiroyuki; Xiao, Hua; Qin, Jun; Vahlne, Anders; Roeder, Robert G.

In: Journal of Biomedical Science, Vol. 9, No. 3, 2002, p. 234-245.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Estable, Mario Clemente

AU - Naghavi, Mojgan H.

AU - Kato, Hiroyuki

AU - Xiao, Hua

AU - Qin, Jun

AU - Vahlne, Anders

AU - Roeder, Robert G.

N1 - Funding Information: The authors thank M. Guermah, S. Malik and V. Palhan fbr advice throughout this work and S.M., M.G. and M. Teichmann tbr careful reading of the manuscript. M.C.E. is a Fellow of the Canadian Institutes of Health Research (formerly MRC). "['his work was supported by NIH grant AI37327-05 to R.G.R.

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N2 - Positive transcription elongation factor-b (P-TEFb) contains CDK9 and cyclin T1. P-TEFb was affinity purified from a stably transfected cell line that expresses epitope-tagged CDK9, and proteins that appeared to be specifically bound were sequenced. In addition to CDK9, previously identified isoforms of cyclin T (including T1, T2A and T2B), HSP90 and CDC37, this analysis identified a novel protein named MCEF. Cloning of its cognate cDNA revealed that MCEF is the newest member of the AF4 family of transcription factors involved in acute lymphoblastic leukemia. MCEF RNA was expressed in all human tissues examined, and antisera directed against recombinant MCEF specifically immunoprecipitated P-TEFb. Ectopic expression of MCEF did not activate HIV-1 replication, and tethering of MCEF to a promoter did not activate transcription.

AB - Positive transcription elongation factor-b (P-TEFb) contains CDK9 and cyclin T1. P-TEFb was affinity purified from a stably transfected cell line that expresses epitope-tagged CDK9, and proteins that appeared to be specifically bound were sequenced. In addition to CDK9, previously identified isoforms of cyclin T (including T1, T2A and T2B), HSP90 and CDC37, this analysis identified a novel protein named MCEF. Cloning of its cognate cDNA revealed that MCEF is the newest member of the AF4 family of transcription factors involved in acute lymphoblastic leukemia. MCEF RNA was expressed in all human tissues examined, and antisera directed against recombinant MCEF specifically immunoprecipitated P-TEFb. Ectopic expression of MCEF did not activate HIV-1 replication, and tethering of MCEF to a promoter did not activate transcription.

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MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein

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