MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein

Mario Clemente Estable, Mojgan H. Naghavi, Hiroyuki Kato, Hua Xiao, Jun Qin, Anders Vahlne, Robert G. Roeder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Positive transcription elongation factor-b (P-TEFb) contains CDK9 and cyclin T1. P-TEFb was affinity purified from a stably transfected cell line that expresses epitope-tagged CDK9, and proteins that appeared to be specifically bound were sequenced. In addition to CDK9, previously identified isoforms of cyclin T (including T1, T2A and T2B), HSP90 and CDC37, this analysis identified a novel protein named MCEF. Cloning of its cognate cDNA revealed that MCEF is the newest member of the AF4 family of transcription factors involved in acute lymphoblastic leukemia. MCEF RNA was expressed in all human tissues examined, and antisera directed against recombinant MCEF specifically immunoprecipitated P-TEFb. Ectopic expression of MCEF did not activate HIV-1 replication, and tethering of MCEF to a promoter did not activate transcription.

Original languageEnglish (US)
Pages (from-to)234-245
Number of pages12
JournalJournal of Biomedical Science
Volume9
Issue number3
DOIs
StatePublished - 2002

Keywords

  • CDK9
  • HIV
  • Leukemia
  • Positive transcription elongation factor-b
  • Transcription

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein'. Together they form a unique fingerprint.

Cite this