MCT-1 protein interacts with the cap complex and modulates messenger RNA translational profiles

Line S. Reinert, Bo Shi, Suvobroto Nandi, Krystyna Mazan-Mamczarz, Michele Vitolo, Kurtis E. Bachman, Huili He, Ronald B. Gartenhaus*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

MCT-1 is an oncogene that was initially identified in a human T cell lymphoma and has been shown to induce cell proliferation as well as activate survival-related pathways. MCT-1 contains the PUA domain, a recently described RNA-binding domain that is found in several tRNA and rRNA modification enzymes. Here, we established that MCT-1 protein interacts with the cap complex through its PUA domain and recruits the density-regulated protein (DENR/DRP), containing the SUI1 translation initiation domain. Through the use of microarray analysis on polysome-associated mRNAs, we showed that up-regulation of MCT-1 was able to modulate the translation profiles of BCL2L2, TFDP1, MRE11A, cyclin D1, and E2F1 mRNAs, despite equivalent levels of mRNAs in the cytoplasm. Our data establish a role for MCT-1 in translational regulation, and support a linkage between translational control and oncogenesis.

Original languageEnglish (US)
Pages (from-to)8994-9001
Number of pages8
JournalCancer Research
Volume66
Issue number18
DOIs
StatePublished - Sep 15 2006

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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