TY - JOUR
T1 - MCT-1 protein interacts with the cap complex and modulates messenger RNA translational profiles
AU - Reinert, Line S.
AU - Shi, Bo
AU - Nandi, Suvobroto
AU - Mazan-Mamczarz, Krystyna
AU - Vitolo, Michele
AU - Bachman, Kurtis E.
AU - He, Huili
AU - Gartenhaus, Ronald B.
PY - 2006/9/15
Y1 - 2006/9/15
N2 - MCT-1 is an oncogene that was initially identified in a human T cell lymphoma and has been shown to induce cell proliferation as well as activate survival-related pathways. MCT-1 contains the PUA domain, a recently described RNA-binding domain that is found in several tRNA and rRNA modification enzymes. Here, we established that MCT-1 protein interacts with the cap complex through its PUA domain and recruits the density-regulated protein (DENR/DRP), containing the SUI1 translation initiation domain. Through the use of microarray analysis on polysome-associated mRNAs, we showed that up-regulation of MCT-1 was able to modulate the translation profiles of BCL2L2, TFDP1, MRE11A, cyclin D1, and E2F1 mRNAs, despite equivalent levels of mRNAs in the cytoplasm. Our data establish a role for MCT-1 in translational regulation, and support a linkage between translational control and oncogenesis.
AB - MCT-1 is an oncogene that was initially identified in a human T cell lymphoma and has been shown to induce cell proliferation as well as activate survival-related pathways. MCT-1 contains the PUA domain, a recently described RNA-binding domain that is found in several tRNA and rRNA modification enzymes. Here, we established that MCT-1 protein interacts with the cap complex through its PUA domain and recruits the density-regulated protein (DENR/DRP), containing the SUI1 translation initiation domain. Through the use of microarray analysis on polysome-associated mRNAs, we showed that up-regulation of MCT-1 was able to modulate the translation profiles of BCL2L2, TFDP1, MRE11A, cyclin D1, and E2F1 mRNAs, despite equivalent levels of mRNAs in the cytoplasm. Our data establish a role for MCT-1 in translational regulation, and support a linkage between translational control and oncogenesis.
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UR - http://www.scopus.com/inward/citedby.url?scp=33749489960&partnerID=8YFLogxK
U2 - 10.1158/0008-5472.CAN-06-1999
DO - 10.1158/0008-5472.CAN-06-1999
M3 - Article
C2 - 16982740
AN - SCOPUS:33749489960
SN - 0008-5472
VL - 66
SP - 8994
EP - 9001
JO - Cancer Research
JF - Cancer Research
IS - 18
ER -
