Measurement of hypocretin/orexin content in the mouse brain using an enzyme immunoassay: The effect of circadian time, age and genetic background

L. Lin, J. Wisor, T. Shiba, S. Taheri, K. Yanai, S. Wurts, X. Lin, M. Vitaterna, J. Takahashi, T. W. Lovenberg, M. Koehl, G. Uhl, S. Nishino, E. Mignot*

*Corresponding author for this work

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

The hypocretins (1 and 2) have emerged as key regulators of sleep and wakefulness. We developed a high-throughput enzyme immunoassay (EIA) to measure total brain hypocretin levels from large numbers of mice. Hypocretin levels were not altered by circadian time or age. However, significant differences in one or both hypocretin peptides were observed between different mouse strains. We studied hypocretin levels in knockout and transgenic mouse models with obesity, circadian gene mutations or monoaminergic defects. Compared to controls, only histamine receptor knockouts had lower hypocretin levels. This was most pronounced in H1 receptor knockouts suggesting the existence of a positive feedback loop between hypocretin and histaminergic neurons.

Original languageEnglish (US)
Pages (from-to)2203-2211
Number of pages9
JournalPeptides
Volume23
Issue number12
DOIs
StatePublished - Dec 1 2002

Keywords

  • Enzyme immunoassay
  • Histamine receptor
  • Hypocretin
  • Knockout
  • Narcolepsy
  • Orexin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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    Lin, L., Wisor, J., Shiba, T., Taheri, S., Yanai, K., Wurts, S., Lin, X., Vitaterna, M., Takahashi, J., Lovenberg, T. W., Koehl, M., Uhl, G., Nishino, S., & Mignot, E. (2002). Measurement of hypocretin/orexin content in the mouse brain using an enzyme immunoassay: The effect of circadian time, age and genetic background. Peptides, 23(12), 2203-2211. https://doi.org/10.1016/S0196-9781(02)00251-6