Abstract
The development of the controlled potential method for studying vivo redox reactions is described by showing some of the results of its first application to photosynthetic systems. Phototrap activity was shown to be operative only within the potential range from -0.I to + 0.5 V in R. rubrum and R. Spheroides. New results obtained by applying the technique to a complex enzymatic system, photophosphorylation, have shown that this activity is shut off at lower potential in parallel to shutting off phototrap activity. Optimal activity was found near o V while the activity was again totally quenched at higher potential with a midpoint of 0.30 V. This latter transition may reflect a component at the coupling site for electron transport and phosphorylation. A consideration of the possible effect of conformational changes on U′o values is given and valid usage of the controlled potential method is underscored.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 427-437 |
| Number of pages | 11 |
| Journal | Bioelectrochemistry and Bioenergetics |
| Volume | 1 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - Dec 1974 |
Funding
This research was supported by research grants from the National Institutes of Health (GM 11741) and the National Science Foundation (GB 18420), and a Research Career Development Award from the National Institutes of Health (5 K04 GM 70133).
ASJC Scopus subject areas
- Biophysics
- Physical and Theoretical Chemistry
- Electrochemistry