Measurement of oxidized 5-methyltetrahydrofolic acid in human plasma: A potential biological marker of in vivo oxidation

Vincent P. Flanagan*, Paul M. Thomas, Robert J. Pawlosky

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A stable isotope LC-MS procedure developed to determine the concentration of Mefox in human plasma while monitoring 5-methyltetrahydrofolic acid monoglutamate (5-MTHFA) using an alternate LC-MS method was discussed. Twenty-three plasma samples from smokers and nonsmokers drawn prior to 1995 (before folic acid fortification of foods) were analyzed for 5-MTHFA and Mefox. MRM was used to find Mefox to be present in only one of the plasmas analyzed at a concentration of 23.7 ng/ml. The range for 5-MTHFA was found to be 1.1 to 13.6 ng/ml with a mean of 3.5 ng/ml.

Original languageEnglish (US)
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages551-552
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Flanagan, V. P., Thomas, P. M., & Pawlosky, R. J. (2002). Measurement of oxidized 5-methyltetrahydrofolic acid in human plasma: A potential biological marker of in vivo oxidation. In Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 551-552)