Abstract
Protein-ligand dissociation constants, Kd, are determined precisely and down to the picomolar range from reaction-diffusion (RD) concentration profiles created by proteins diffusing through hydrogels functionalized with protein ligands. The RD process effectively amplifies the molecular-scale binding events into macroscopic patterns visible to the naked eye. The method is applicable to various protein-ligand pairs and does not require any prior knowledge about the protein structure.
Original language | English (US) |
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Pages (from-to) | 8780-8784 |
Number of pages | 5 |
Journal | Analytical Chemistry |
Volume | 82 |
Issue number | 21 |
DOIs | |
State | Published - Nov 1 2010 |
ASJC Scopus subject areas
- Analytical Chemistry