Measurement of protein-ligand binding constants from reaction-diffusion concentration profiles

Yanhu Wei, Paul J. Wesson, Igor Kourkine, Bartosz A. Grzybowski

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Protein-ligand dissociation constants, Kd, are determined precisely and down to the picomolar range from reaction-diffusion (RD) concentration profiles created by proteins diffusing through hydrogels functionalized with protein ligands. The RD process effectively amplifies the molecular-scale binding events into macroscopic patterns visible to the naked eye. The method is applicable to various protein-ligand pairs and does not require any prior knowledge about the protein structure.

Original languageEnglish (US)
Pages (from-to)8780-8784
Number of pages5
JournalAnalytical Chemistry
Volume82
Issue number21
DOIs
StatePublished - Nov 1 2010

ASJC Scopus subject areas

  • Analytical Chemistry

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