Measuring cellular structure at submicrometer scale with light scattering spectroscopy

Vadim Backman; Venkatesh Gopal; Maxim Kalashnikov; Kamran Badizadegan; Rajan Gurjar; Adam Wax; Irene Georgakoudi; Markus Mueller; Charles W. Boone; Ramachandra R. Dasari; Michael S. Feld

doi:10.1109/2944.983289

Measuring cellular structure at submicrometer scale with light scattering spectroscopy

Vadim Backman^*, Venkatesh Gopal, Maxim Kalashnikov, Kamran Badizadegan, Rajan Gurjar, Adam Wax, Irene Georgakoudi, Markus Mueller, Charles W. Boone, Ramachandra R. Dasari, Michael S. Feld

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

137 Scopus citations

Abstract

We present a novel instrument for imaging the angular distributions of light backscattered by biological cells and tissues. The intensities in different regions of the image are due to scatterers of different sizes. We exploit this to study scattering from particles smaller than the wavelength of light used, even when they are mixed with larger particles. We show that the scattering from subcellular structure in both normal and cancerous human cells is best fitted to inverse power-law distributions for the sizes of the scattering objects, and propose that the distribution of scattering objects may be different in normal versus cancerous cells.

Original language	English (US)
Pages (from-to)	887-893
Number of pages	7
Journal	IEEE Journal on Selected Topics in Quantum Electronics
Volume	7
Issue number	6
DOIs	https://doi.org/10.1109/2944.983289
State	Published - Nov 2001

Funding

Manuscript received August 10, 2001; revised October 30, 2001. This work was supported by NIH Grant P41-RR02594 and by NIH Grant R01-CA53717. V. Backman is with the Biomedical Engineering Department, Northwestern University, Evanston, IL 60208 USA. V. Gopal, M. Kalashnikov, R. Gurjar, A. Wax, I. Georgakoudi, M. Mueller, C. W. Boone, R. R. Dasari, and M. S. Feld are with the G.R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139 USA. K. Badizadegan is with the Department of Pathology, Children’s Hospital, Harvard Medical School, Boston, MA 02115 USA. Publisher Item Identifier S 1077-260X(01)11252-9.

Keywords

Cancer diagnosis
Chromatin
Fractal
Light scattering
Spectroscopy

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/2944.983289

Cite this

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title = "Measuring cellular structure at submicrometer scale with light scattering spectroscopy",

abstract = "We present a novel instrument for imaging the angular distributions of light backscattered by biological cells and tissues. The intensities in different regions of the image are due to scatterers of different sizes. We exploit this to study scattering from particles smaller than the wavelength of light used, even when they are mixed with larger particles. We show that the scattering from subcellular structure in both normal and cancerous human cells is best fitted to inverse power-law distributions for the sizes of the scattering objects, and propose that the distribution of scattering objects may be different in normal versus cancerous cells.",

keywords = "Cancer diagnosis, Chromatin, Fractal, Light scattering, Spectroscopy",

author = "Vadim Backman and Venkatesh Gopal and Maxim Kalashnikov and Kamran Badizadegan and Rajan Gurjar and Adam Wax and Irene Georgakoudi and Markus Mueller and Boone, {Charles W.} and Dasari, {Ramachandra R.} and Feld, {Michael S.}",

note = "Funding Information: Manuscript received August 10, 2001; revised October 30, 2001. This work was supported by NIH Grant P41-RR02594 and by NIH Grant R01-CA53717. V. Backman is with the Biomedical Engineering Department, Northwestern University, Evanston, IL 60208 USA. V. Gopal, M. Kalashnikov, R. Gurjar, A. Wax, I. Georgakoudi, M. Mueller, C. W. Boone, R. R. Dasari, and M. S. Feld are with the G.R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139 USA. K. Badizadegan is with the Department of Pathology, Children{\textquoteright}s Hospital, Harvard Medical School, Boston, MA 02115 USA. Publisher Item Identifier S 1077-260X(01)11252-9.",

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doi = "10.1109/2944.983289",

language = "English (US)",

volume = "7",

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AU - Gurjar, Rajan

AU - Wax, Adam

AU - Georgakoudi, Irene

AU - Mueller, Markus

AU - Boone, Charles W.

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

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N2 - We present a novel instrument for imaging the angular distributions of light backscattered by biological cells and tissues. The intensities in different regions of the image are due to scatterers of different sizes. We exploit this to study scattering from particles smaller than the wavelength of light used, even when they are mixed with larger particles. We show that the scattering from subcellular structure in both normal and cancerous human cells is best fitted to inverse power-law distributions for the sizes of the scattering objects, and propose that the distribution of scattering objects may be different in normal versus cancerous cells.

AB - We present a novel instrument for imaging the angular distributions of light backscattered by biological cells and tissues. The intensities in different regions of the image are due to scatterers of different sizes. We exploit this to study scattering from particles smaller than the wavelength of light used, even when they are mixed with larger particles. We show that the scattering from subcellular structure in both normal and cancerous human cells is best fitted to inverse power-law distributions for the sizes of the scattering objects, and propose that the distribution of scattering objects may be different in normal versus cancerous cells.

KW - Cancer diagnosis

KW - Chromatin

KW - Fractal

KW - Light scattering

KW - Spectroscopy

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Measuring cellular structure at submicrometer scale with light scattering spectroscopy

Abstract

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Keywords

ASJC Scopus subject areas

UN SDGs

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