Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy

C. Monzel; D. Schmidt; C. Kleusch; D. Kirchenbüchler; U. Seifert; A. S. Smith; K. Sengupta; R. Merkel

doi:10.1038/ncomms9162

Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy

C. Monzel, D. Schmidt, C. Kleusch, D. Kirchenbüchler, U. Seifert, A. S. Smith, K. Sengupta, R. Merkel^*

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Stochastic displacements or fluctuations of biological membranes are increasingly recognized as an important aspect of many physiological processes, but hitherto their precise quantification in living cells was limited due to a lack of tools to accurately record them. Here we introduce a novel technique-dynamic optical displacement spectroscopy (DODS), to measure stochastic displacements of membranes with unprecedented combined spatiotemporal resolution of 20'nm and 10'Î 1/4s. The technique was validated by measuring bending fluctuations of model membranes. DODS was then used to explore the fluctuations in human red blood cells, which showed an ATP-induced enhancement of non-Gaussian behaviour. Plasma membrane fluctuations of human macrophages were quantified to this accuracy for the first time. Stimulation with a cytokine enhanced non-Gaussian contributions to these fluctuations. Simplicity of implementation, and high accuracy make DODS a promising tool for comprehensive understanding of stochastic membrane processes.

Original language	English (US)
Article number	8162
Journal	Nature communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms9162
State	Published - Oct 6 2015

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/ncomms9162

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title = "Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy",

abstract = "Stochastic displacements or fluctuations of biological membranes are increasingly recognized as an important aspect of many physiological processes, but hitherto their precise quantification in living cells was limited due to a lack of tools to accurately record them. Here we introduce a novel technique-dynamic optical displacement spectroscopy (DODS), to measure stochastic displacements of membranes with unprecedented combined spatiotemporal resolution of 20'nm and 10'{\^I} 1/4s. The technique was validated by measuring bending fluctuations of model membranes. DODS was then used to explore the fluctuations in human red blood cells, which showed an ATP-induced enhancement of non-Gaussian behaviour. Plasma membrane fluctuations of human macrophages were quantified to this accuracy for the first time. Stimulation with a cytokine enhanced non-Gaussian contributions to these fluctuations. Simplicity of implementation, and high accuracy make DODS a promising tool for comprehensive understanding of stochastic membrane processes.",

author = "C. Monzel and D. Schmidt and C. Kleusch and D. Kirchenb{\"u}chler and U. Seifert and Smith, {A. S.} and K. Sengupta and R. Merkel",

note = "Funding Information: We thank Nils Hersch, Victor Messerschmidt and Tim Urbansky for providing macrophages. C.M. is grateful for the financial support by UFA and Cellnetworks. We thank David Gachet and Susanne Fenz for fruitful discussions and Laurent Limozin for careful reading of the manuscript. K.S. thanks European Research Council for funding via grant no. 307104 FP/2007-2013/ERC/SYNINTER and ASS for ERC/StG 337283. Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited.",

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AU - Monzel, C.

AU - Schmidt, D.

AU - Kleusch, C.

AU - Kirchenbüchler, D.

AU - Seifert, U.

AU - Smith, A. S.

AU - Sengupta, K.

AU - Merkel, R.

N1 - Funding Information: We thank Nils Hersch, Victor Messerschmidt and Tim Urbansky for providing macrophages. C.M. is grateful for the financial support by UFA and Cellnetworks. We thank David Gachet and Susanne Fenz for fruitful discussions and Laurent Limozin for careful reading of the manuscript. K.S. thanks European Research Council for funding via grant no. 307104 FP/2007-2013/ERC/SYNINTER and ASS for ERC/StG 337283. Publisher Copyright: © 2015 Macmillan Publishers Limited.

PY - 2015/10/6

Y1 - 2015/10/6

N2 - Stochastic displacements or fluctuations of biological membranes are increasingly recognized as an important aspect of many physiological processes, but hitherto their precise quantification in living cells was limited due to a lack of tools to accurately record them. Here we introduce a novel technique-dynamic optical displacement spectroscopy (DODS), to measure stochastic displacements of membranes with unprecedented combined spatiotemporal resolution of 20'nm and 10'Î 1/4s. The technique was validated by measuring bending fluctuations of model membranes. DODS was then used to explore the fluctuations in human red blood cells, which showed an ATP-induced enhancement of non-Gaussian behaviour. Plasma membrane fluctuations of human macrophages were quantified to this accuracy for the first time. Stimulation with a cytokine enhanced non-Gaussian contributions to these fluctuations. Simplicity of implementation, and high accuracy make DODS a promising tool for comprehensive understanding of stochastic membrane processes.

AB - Stochastic displacements or fluctuations of biological membranes are increasingly recognized as an important aspect of many physiological processes, but hitherto their precise quantification in living cells was limited due to a lack of tools to accurately record them. Here we introduce a novel technique-dynamic optical displacement spectroscopy (DODS), to measure stochastic displacements of membranes with unprecedented combined spatiotemporal resolution of 20'nm and 10'Î 1/4s. The technique was validated by measuring bending fluctuations of model membranes. DODS was then used to explore the fluctuations in human red blood cells, which showed an ATP-induced enhancement of non-Gaussian behaviour. Plasma membrane fluctuations of human macrophages were quantified to this accuracy for the first time. Stimulation with a cytokine enhanced non-Gaussian contributions to these fluctuations. Simplicity of implementation, and high accuracy make DODS a promising tool for comprehensive understanding of stochastic membrane processes.

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Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy

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