Abstract
Visible light optical coherence tomography (vis-OCT) has demonstrated its capability of measuring vascular oxygen saturation (sO2) in vivo. Enhanced by OCT angiography, the signal from microvasculature can be further isolated and directly used for sO2 extraction. In this work, we extended the theoretical formulation for OCT angiography-based oximetry by incorporating the contribution from motion contrast enhancement. We presented a new method to eliminate the associated confounding variables due to blood flow. First, we performed in vitro experiments to verify our theory, showing a stable spectral derivative within the selected wavelength bands for sO2 extraction. Then, we tested our method in vivo to measure retinal sO2 in rats inhaling different gas mixtures: normal air, 5% CO2, pure O2, and 10% O2. Absolute sO2 values in major arterioles and venules in the retinal circulation can be accurately measured. In addition, we demonstrated the relative changes of sO2 can be measured non-invasively from choriocapillaris immediately underneath the retinal pigmented epithelium (RPE) in rodents.
Original language | English (US) |
---|---|
Pages (from-to) | 2840-2853 |
Number of pages | 14 |
Journal | Biomedical Optics Express |
Volume | 6 |
Issue number | 8 |
DOIs | |
State | Published - Jul 10 2015 |
Keywords
- Blood gas monitoring
- Functional monitoring and imaging
- Optical coherence tomography
- Speckle
- Spectroscopy
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Biotechnology
Fingerprint
Dive into the research topics of 'Measuring oxygen saturation in retinal and choroidal circulations in rats using visible light optical coherence tomography angiography'. Together they form a unique fingerprint.Datasets
-
Measuring oxygen saturation in retinal and choroidal circulations in rats using visible light optical coherence tomography angiography
Chen, S. (Contributor), Yi, J. (Creator) & Zhang, H. F. (Creator), figshare, 2015
DOI: 10.6084/m9.figshare.c.3752948.v1, https://figshare.com/collections/Measuring_oxygen_saturation_in_retinal_and_choroidal_circulations_in_rats_using_visible_light_optical_coherence_tomography_angiography/3752948/1
Dataset
-
Measuring oxygen saturation in retinal and choroidal circulations in rats using visible light optical coherence tomography angiography
Chen, S. (Contributor), Yi, J. (Creator) & Zhang, H. F. (Creator), figshare, 2015
DOI: 10.6084/m9.figshare.c.3752948, https://figshare.com/collections/Measuring_oxygen_saturation_in_retinal_and_choroidal_circulations_in_rats_using_visible_light_optical_coherence_tomography_angiography/3752948
Dataset