Mechanism of inactivation of γ-aminobutyric acid aminotransferase by (S,E)-4-amino-5-fluoropent-2-enoic acid

Richard B. Silverman*, Cherian George

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Evidence for an enamine mechanism of inactivation of pig brain γ-aminobutyric acid (GABA) aminotransferase by (S,E)-4-amino-5-fluoropent-2-enoic acid is presented. apo-GABA aminotransferase reconstituted with [3H]pyridoxal 5′-phosphate is inactivated by (S,E)-4-amino-5-fluoropent-2-enoic acid and the pH is raised to 12. All of the radioactivity is released from the enzyme as an adduct of the cofactor; no [3H]pyridoxamine 5′-phosphate is generated.

Original languageEnglish (US)
Pages (from-to)942-946
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume150
Issue number3
DOIs
StatePublished - Feb 15 1988

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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