TY - JOUR
T1 - Mechanism of inactivation of γ-aminobutyric acid aminotransferase by (S,E)-4-amino-5-fluoropent-2-enoic acid
AU - Silverman, Richard B.
AU - George, Cherian
N1 - Funding Information:
We are grateful to the National Institutes of Health (grant NS 15703) financial support of this work. R.B.S. is the recipient of a NIH Research Career Development Award (1982-1987). The authors wish to thank Dr. Jacob Mathew for synthesizing 4.
PY - 1988/2/15
Y1 - 1988/2/15
N2 - Evidence for an enamine mechanism of inactivation of pig brain γ-aminobutyric acid (GABA) aminotransferase by (S,E)-4-amino-5-fluoropent-2-enoic acid is presented. apo-GABA aminotransferase reconstituted with [3H]pyridoxal 5′-phosphate is inactivated by (S,E)-4-amino-5-fluoropent-2-enoic acid and the pH is raised to 12. All of the radioactivity is released from the enzyme as an adduct of the cofactor; no [3H]pyridoxamine 5′-phosphate is generated.
AB - Evidence for an enamine mechanism of inactivation of pig brain γ-aminobutyric acid (GABA) aminotransferase by (S,E)-4-amino-5-fluoropent-2-enoic acid is presented. apo-GABA aminotransferase reconstituted with [3H]pyridoxal 5′-phosphate is inactivated by (S,E)-4-amino-5-fluoropent-2-enoic acid and the pH is raised to 12. All of the radioactivity is released from the enzyme as an adduct of the cofactor; no [3H]pyridoxamine 5′-phosphate is generated.
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U2 - 10.1016/0006-291X(88)90720-6
DO - 10.1016/0006-291X(88)90720-6
M3 - Article
C2 - 3342072
AN - SCOPUS:0023872156
SN - 0006-291X
VL - 150
SP - 942
EP - 946
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -