Mechanism of Inactivation of GABA Aminotransferase by (E)- and (Z)-(1S,3S)-3-Amino-4-fluoromethylenyl-1-cyclopentanoic Acid

Hyunbeom Lee, Hoang V. Le, Rui Wu, Emma Doud, Ruslan Sanishvili, John F. Kellie, Philip D Compton, Boobalan Pachaiyappan, Dali Liu, Neil L Kelleher, Richard B Silverman*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

When γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the mammalian central nervous system, falls below a threshold level, seizures occur. One approach to raise GABA concentrations is to inhibit GABA aminotransferase (GABA-AT), a pyridoxal 5′-phosphate-dependent enzyme that degrades GABA. We have previously developed (1S,3S)-3-amino-4-difluoromethylene-1-cyclopentanoic acid (CPP-115), which is 186 times more efficient in inactivating GABA-AT than vigabatrin, the only FDA-approved inactivator of GABA-AT. We also developed (E)- and (Z)-(1S,3S)-3-amino-4-fluoromethylenyl-1-cyclopentanoic acid (1 and 2, respectively), monofluorinated analogs of CPP-115, which are comparable to vigabatrin in inactivating GABA-AT. Here, we report the mechanism of inactivation of GABA-AT by 1 and 2. Both produce a metabolite that induces disruption of the Glu270-Arg445 salt bridge to accommodate interaction between the metabolite formyl group and Arg445. This is the second time that Arg445 has interacted with a ligand and is involved in GABA-AT inactivation, thereby confirming the importance of Arg445 in future inactivator design.

Original languageEnglish (US)
Pages (from-to)2087-2098
Number of pages12
JournalACS chemical biology
Volume10
Issue number9
DOIs
StatePublished - Sep 18 2015

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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