Treatment of normal BALB/c spleen cell cultures with antibodies specific for the idiotype of the TEPC-15 myeloma protein (anti-T15id) suppressed the induction of anti-phosphorylcholine (PC) antibody by either antigen or polyclonal B cell activators (PBA). Preincubation of cultures with anti-T15id antibody was necessary to suppress the lipopolysaccharide- (LPS) stimulated anti-PC response, whereas such preincubation was not necessary for suppression of the antigen-induced anti-PC response. The duration of preincubation with anti-T15id antibody required to suppress the LPS-induced anti-PC response appeared to be inversely proportional to the concentration of the anti-T15id antibody. Similar preincubations at low temperature (0°C) did not result in suppression of the LPS-inducible anti-PC response, suggesting that modulation of the antigen receptors by anti-idiotype antibody is necessary for suppression. However, without a preincubation with anti-T15id antibody, a 100-fold increase in the concentration of the anti-T15id antibody was required to attain a level of suppression similar to the antigen-induced anti-PC response. Suppression of the anti-PC response induced by other PBA (dextran sulfate and pokeweed mitogen) also required a similar preincubation with anti-T15id antibody, implying a common pathway for inactivation of PBA-stimulated cultures that is distinct from inactivation of the antigen-induced response. Since elimination of the cells that had been stimulated by either antigen or LPS by treatment with 5-bromodeoxyuridine resulted in complete tolerance to antigen and LPS, the difference in susceptibility to anti-T15id antibody is not likely due to the responsive cell populations. Therefore, this difference in sensitivity to anti-idiotype antibody may reflect the presence of 2 distinct pathways for B cell activation; perhaps the signal(s) of PBA for B cell differentiation is more direct and/or stronger than that of specific antigen.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Immunology|
|State||Published - 1981|
ASJC Scopus subject areas
- Immunology and Allergy