Megakaryocyte polyploidization and proplatelet formation in low-attachment conditions

Alaina C. Schlinker, Mark T. Duncan, Teresa A. DeLuca, David C. Whitehead, William M. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In vitro-derived platelets (PLTs), which could provide an alternative source of PLTs for patient transfusions, are formed from polyploid megakaryocytes (MKs) that extend long cytoplasmic projections, termed proplatelets (proPLTs). In this study, we compared polyploidization and proPLT formation (PPF) of MKs cultured on surfaces that either promote or inhibit protein adsorption and subsequent cell adhesion. A megakaryoblastic cell line exhibited increased polyploidization and arrested PPF on a low-attachment surface. Primary human MKs also showed low levels of PPF on the same surface, but no difference in ploidy. Importantly, both cell types exhibited accelerated PPF after transfer to a surface that supports attachment, suggesting that pre-culture on a non-adhesive surface may facilitate synchronization of PPF and PLT generation in culture.

Original languageEnglish (US)
Pages (from-to)24-33
Number of pages10
JournalBiochemical Engineering Journal
Volume111
DOIs
StatePublished - Jul 15 2016

Keywords

  • Animal cell culture
  • Biomedical
  • Cell adhesion
  • Culture-derived platelets
  • Physiology
  • Tissue culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

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