TY - JOUR
T1 - MEK7-dependent Activation of p38 MAP Kinase in Keratinocytes
AU - Dashti, Shervin R.
AU - Efimova, Tatiana
AU - Eckert, Richard L.
PY - 2001/3/16
Y1 - 2001/3/16
N2 - Previous studies suggest that a PKC/Ras/MEKK1 cascade regulates involucrin (hINV) gene expression in human epidermal keratinocytes. MEK7, which is expressed in epidermis, has been identified as a member of this cascade (Efimova, T., LaCelle, P., Welter, J. F., and Eckert, R. L. (1998) J. Biol. Chem. 273, 24387-24395 and Efimova, T., and Eckert, R. L. (2000) J. Biol. Chem. 275, 1601-1607). However, the kinase that functions downstream of MEK7 has not been identified. Our present studies show that MEK7 expression in keratinocytes markedly activates p38α and modestly activates JNK. Activation of p38 MAPK by MEK7 is a novel finding, as previous reports have assigned MEK7 as a JNK regulator. We also demonstrate that this regulation is physiologically important, as the p38α- and JNK-dependent activities regulate hINV promoter activity and expression of the endogenous hINV gene.
AB - Previous studies suggest that a PKC/Ras/MEKK1 cascade regulates involucrin (hINV) gene expression in human epidermal keratinocytes. MEK7, which is expressed in epidermis, has been identified as a member of this cascade (Efimova, T., LaCelle, P., Welter, J. F., and Eckert, R. L. (1998) J. Biol. Chem. 273, 24387-24395 and Efimova, T., and Eckert, R. L. (2000) J. Biol. Chem. 275, 1601-1607). However, the kinase that functions downstream of MEK7 has not been identified. Our present studies show that MEK7 expression in keratinocytes markedly activates p38α and modestly activates JNK. Activation of p38 MAPK by MEK7 is a novel finding, as previous reports have assigned MEK7 as a JNK regulator. We also demonstrate that this regulation is physiologically important, as the p38α- and JNK-dependent activities regulate hINV promoter activity and expression of the endogenous hINV gene.
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U2 - 10.1074/jbc.C000862200
DO - 10.1074/jbc.C000862200
M3 - Article
C2 - 11244091
AN - SCOPUS:0035896611
SN - 0021-9258
VL - 276
SP - 8059
EP - 8063
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 11
ER -