@article{9f00b6e97cdc4412821bb1018a73deff,
title = "Melanopsin Retinal Ganglion Cells Regulate Cone Photoreceptor Lamination in the Mouse Retina",
abstract = "Newborn neurons follow molecular cues to reach their final destination, but whether early life experience influences lamination remains largely unexplored. As light is among the first stimuli to reach the developing nervous system via intrinsically photosensitive retinal ganglion cells (ipRGCs), we asked whether ipRGCs could affect lamination in the developing mouse retina. We show here that ablation of ipRGCs causes cone photoreceptors to mislocalize at different apicobasal positions in the retina. This effect is partly mediated by light-evoked activity in ipRGCs, as dark rearing or silencing of ipRGCs leads a subset of cones to mislocalize. Furthermore, ablation of ipRGCs alters the cone transcriptome and decreases expression of the dopamine receptor D4, while injection of L-DOPA or D4 receptor agonist rescues the displaced cone phenotype observed in dark-reared animals. These results show that early light-mediated activity in ipRGCs influences neuronal lamination and identify ipRGC-elicited dopamine release as a mechanism influencing cone position. Tufford et al. show that light acting through intrinsically photosensitive retinal ganglion cells (ipRGCs) controls cone photoreceptor positioning in the developing mouse retina, using dopamine signaling as a cue. Ablation of ipRGCs disrupts cone positioning and induces changes in the cone transcriptome.",
keywords = "cell migration, cone photoreceptors, development, dopamine, intrinsically photosensitive retinal ganglion cells, lamination, melanopsin, mouse, neuronal layers, retina",
author = "Tufford, {Adele R.} and Onyak, {Jessica R.} and Sondereker, {Katelyn B.} and Lucas, {Jasmine A.} and Earley, {Aaron M.} and Pierre Mattar and Samer Hattar and Schmidt, {Tiffany M.} and Renna, {Jordan M.} and Michel Cayouette",
note = "Funding Information: We wish to thank B. Day, M.C. Lavall{\'e}e, and J. Barthe for mouse care; M. Goulding for the Rosa TeNT mice; E. Ruthazer, K. Murai, and G. Awatramani for critical insight on the project; and members of the Cayouette lab for support. This work was supported by grants from the Foundation Fighting Blindness Canada and the Canadian Institutes of Health Research (CIHR 77570 ). A.R.T. was supported by a CIHR Canada Graduate Scholarship . M.C. holds the Ga{\"e}tane and Roland Pilleni{\`e}re Chair in Retina Biology and is an Emeritus Research Scholar from the Fonds de la Recherche – Qu{\'e}bec (FRQS). Additional support to J.M.R. was provided by the NIH ( R15EY026255-01 ) and The Karl Kirchgessner Foundation . Funding Information: We wish to thank B. Day, M.C. Lavall?e, and J. Barthe for mouse care; M. Goulding for the RosaTeNT mice; E. Ruthazer, K. Murai, and G. Awatramani for critical insight on the project; and members of the Cayouette lab for support. This work was supported by grants from the Foundation Fighting Blindness Canada and the Canadian Institutes of Health Research (CIHR 77570). A.R.T. was supported by a CIHR Canada Graduate Scholarship. M.C. holds the Ga?tane and Roland Pilleni?re Chair in Retina Biology and is an Emeritus Research Scholar from the Fonds de la Recherche ? Qu?bec (FRQS). Additional support to J.M.R. was provided by the NIH (R15EY026255-01) and The Karl Kirchgessner Foundation. Publisher Copyright: {\textcopyright} 2018 The Author(s)",
year = "2018",
month = may,
day = "22",
doi = "10.1016/j.celrep.2018.04.086",
language = "English (US)",
volume = "23",
pages = "2416--2428",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "8",
}