Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps

Valeria Levi, Vladimir I. Gelfand, Anna S. Serpinskaya, Enrico Gratton*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly the melanosomes' motion.

Original languageEnglish (US)
Pages (from-to)L07-L09
JournalBiophysical Journal
Volume90
Issue number1
DOIs
StatePublished - Jan 2006

ASJC Scopus subject areas

  • Biophysics

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