Membrane trafficking of AQP5 and cAMP dependent phosphorylation in bronchial epithelium

Janghee Woo; Young Kwang Chae; Se Jin Jang; Myoung Sook Kim; Jin Hyen Baek; Jong Chul Park; Barry Trink; Edward Ratovitski; Taekyul Lee; Beomsoo Park; Minjoo Park; Ji Hye Kang; Jean Charles Soria; Juna Lee; Joseph Califano; David Sidransky; Chulso Moon

doi:10.1016/j.bbrc.2007.11.078

Membrane trafficking of AQP5 and cAMP dependent phosphorylation in bronchial epithelium

Janghee Woo, Young Kwang Chae, Se Jin Jang, Myoung Sook Kim, Jin Hyen Baek, Jong Chul Park, Barry Trink, Edward Ratovitski, Taekyul Lee, Beomsoo Park, Minjoo Park, Ji Hye Kang, Jean Charles Soria, Juna Lee, Joseph Califano, David Sidransky, Chulso Moon^*

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Phosphorylation pathway has been identified as an important step in membrane trafficking for AQP5. We generated stably transfected BEAS-2B human bronchial epithelial cells with various over-expression constructs on permeable support. In stable cells with wild-type AQP5 and S156A (AQP5 mutant targeting PKA consensus sequence), AQP5 expression was predominantly polarized to the apical membrane, whereas stable cells with N185D (AQP5 mutant targeting second NPA motif), mainly localized to the cytoplasm. Treatment with H89 and/or chlorophenylthio-cAMP (cpt-cAMP) did not affect membrane expression of AQP5 in any of three stable cells. In cells with wild-type AQP5 and N185D, AQP5s were phosphorylated by PKA, while phosphorylation of AQP5 was not detected in cells with S156A. These results indicate that, in AQP5, serine156 may be phosphorylated by PKA, but membrane expression of AQP5 may not be regulated by PKA phosphorylation. We conclude that AQP5 membrane targeting can include more than one mechanism besides cAMP dependent phosphorylation.

Original language	English (US)
Pages (from-to)	321-327
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	366
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2007.11.078
State	Published - Feb 8 2008

Keywords

AQP5
Aquaporin
Membrane localization
cAMP dependent phosphorylation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2007.11.078

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Woo, J., Chae, Y. K., Jang, S. J., Kim, M. S., Baek, J. H., Park, J. C., Trink, B., Ratovitski, E., Lee, T., Park, B., Park, M., Kang, J. H., Soria, J. C., Lee, J., Califano, J., Sidransky, D., & Moon, C. (2008). Membrane trafficking of AQP5 and cAMP dependent phosphorylation in bronchial epithelium. Biochemical and Biophysical Research Communications, 366(2), 321-327. https://doi.org/10.1016/j.bbrc.2007.11.078

@article{a93b28411c694cf8a3bed5d562e843c3,

title = "Membrane trafficking of AQP5 and cAMP dependent phosphorylation in bronchial epithelium",

abstract = "Phosphorylation pathway has been identified as an important step in membrane trafficking for AQP5. We generated stably transfected BEAS-2B human bronchial epithelial cells with various over-expression constructs on permeable support. In stable cells with wild-type AQP5 and S156A (AQP5 mutant targeting PKA consensus sequence), AQP5 expression was predominantly polarized to the apical membrane, whereas stable cells with N185D (AQP5 mutant targeting second NPA motif), mainly localized to the cytoplasm. Treatment with H89 and/or chlorophenylthio-cAMP (cpt-cAMP) did not affect membrane expression of AQP5 in any of three stable cells. In cells with wild-type AQP5 and N185D, AQP5s were phosphorylated by PKA, while phosphorylation of AQP5 was not detected in cells with S156A. These results indicate that, in AQP5, serine156 may be phosphorylated by PKA, but membrane expression of AQP5 may not be regulated by PKA phosphorylation. We conclude that AQP5 membrane targeting can include more than one mechanism besides cAMP dependent phosphorylation.",

keywords = "AQP5, Aquaporin, Membrane localization, cAMP dependent phosphorylation",

author = "Janghee Woo and Chae, {Young Kwang} and Jang, {Se Jin} and Kim, {Myoung Sook} and Baek, {Jin Hyen} and Park, {Jong Chul} and Barry Trink and Edward Ratovitski and Taekyul Lee and Beomsoo Park and Minjoo Park and Kang, {Ji Hye} and Soria, {Jean Charles} and Juna Lee and Joseph Califano and David Sidransky and Chulso Moon",

note = "Funding Information: The study was supported in part by the SPORE Grant P50-CA96784-01, Cancer Research Grant from Pyung-Ya Foundation (to C.M.), and KOSEF research Grant R01-2004-000-10670-0 and Korea Research Foundation Grant KFR-2004-041-E00064 (to S.J.J.).",

year = "2008",

month = feb,

day = "8",

doi = "10.1016/j.bbrc.2007.11.078",

language = "English (US)",

volume = "366",

pages = "321--327",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

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Woo, J, Chae, YK, Jang, SJ, Kim, MS, Baek, JH, Park, JC, Trink, B, Ratovitski, E, Lee, T, Park, B, Park, M, Kang, JH, Soria, JC, Lee, J, Califano, J, Sidransky, D & Moon, C 2008, 'Membrane trafficking of AQP5 and cAMP dependent phosphorylation in bronchial epithelium', Biochemical and Biophysical Research Communications, vol. 366, no. 2, pp. 321-327. https://doi.org/10.1016/j.bbrc.2007.11.078

TY - JOUR

T1 - Membrane trafficking of AQP5 and cAMP dependent phosphorylation in bronchial epithelium

AU - Woo, Janghee

AU - Chae, Young Kwang

AU - Jang, Se Jin

AU - Kim, Myoung Sook

AU - Baek, Jin Hyen

AU - Park, Jong Chul

AU - Trink, Barry

AU - Ratovitski, Edward

AU - Lee, Taekyul

AU - Park, Beomsoo

AU - Park, Minjoo

AU - Kang, Ji Hye

AU - Soria, Jean Charles

AU - Lee, Juna

AU - Califano, Joseph

AU - Sidransky, David

AU - Moon, Chulso

N1 - Funding Information: The study was supported in part by the SPORE Grant P50-CA96784-01, Cancer Research Grant from Pyung-Ya Foundation (to C.M.), and KOSEF research Grant R01-2004-000-10670-0 and Korea Research Foundation Grant KFR-2004-041-E00064 (to S.J.J.).

PY - 2008/2/8

Y1 - 2008/2/8

N2 - Phosphorylation pathway has been identified as an important step in membrane trafficking for AQP5. We generated stably transfected BEAS-2B human bronchial epithelial cells with various over-expression constructs on permeable support. In stable cells with wild-type AQP5 and S156A (AQP5 mutant targeting PKA consensus sequence), AQP5 expression was predominantly polarized to the apical membrane, whereas stable cells with N185D (AQP5 mutant targeting second NPA motif), mainly localized to the cytoplasm. Treatment with H89 and/or chlorophenylthio-cAMP (cpt-cAMP) did not affect membrane expression of AQP5 in any of three stable cells. In cells with wild-type AQP5 and N185D, AQP5s were phosphorylated by PKA, while phosphorylation of AQP5 was not detected in cells with S156A. These results indicate that, in AQP5, serine156 may be phosphorylated by PKA, but membrane expression of AQP5 may not be regulated by PKA phosphorylation. We conclude that AQP5 membrane targeting can include more than one mechanism besides cAMP dependent phosphorylation.

AB - Phosphorylation pathway has been identified as an important step in membrane trafficking for AQP5. We generated stably transfected BEAS-2B human bronchial epithelial cells with various over-expression constructs on permeable support. In stable cells with wild-type AQP5 and S156A (AQP5 mutant targeting PKA consensus sequence), AQP5 expression was predominantly polarized to the apical membrane, whereas stable cells with N185D (AQP5 mutant targeting second NPA motif), mainly localized to the cytoplasm. Treatment with H89 and/or chlorophenylthio-cAMP (cpt-cAMP) did not affect membrane expression of AQP5 in any of three stable cells. In cells with wild-type AQP5 and N185D, AQP5s were phosphorylated by PKA, while phosphorylation of AQP5 was not detected in cells with S156A. These results indicate that, in AQP5, serine156 may be phosphorylated by PKA, but membrane expression of AQP5 may not be regulated by PKA phosphorylation. We conclude that AQP5 membrane targeting can include more than one mechanism besides cAMP dependent phosphorylation.

KW - AQP5

KW - Aquaporin

KW - Membrane localization

KW - cAMP dependent phosphorylation

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U2 - 10.1016/j.bbrc.2007.11.078

DO - 10.1016/j.bbrc.2007.11.078

M3 - Article

C2 - 18042467

AN - SCOPUS:37449019645

SN - 0006-291X

VL - 366

SP - 321

EP - 327

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Membrane trafficking of AQP5 and cAMP dependent phosphorylation in bronchial epithelium

Abstract

Keywords

ASJC Scopus subject areas

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