Mesodermal Wnt signaling organizes the neural plate via Meis3

Yaniv M. Elkouby, Sarah Elias, Elena S. Casey, Shelby A. Blythe, Nir Tsabar, Peter S. Klein, Heather Root, Karen J. Liu, Dale Frank*

*Corresponding author for this work

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

In vertebrates, canonical Wnt signaling controls posterior neural cell lineage specification. Although Wnt signaling to the neural plate is sufficient for posterior identity, the source and timing of this activity remain uncertain. Furthermore, crucial molecular targets of this activity have not been defined. Here, we identify the endogenous Wnt activity and its role in controlling an essential downstream transcription factor, Meis3. Wnt3a is expressed in a specialized mesodermal domain, the paraxial dorsolateral mesoderm, which signals to overlying neuroectoderm. Loss of zygotic Wnt3a in this region does not alter mesoderm cell fates, but blocks Meis3 expression in the neuroectoderm, triggering the loss of posterior neural fates. Ectopic Meis3 protein expression is sufficient to rescue this phenotype. Moreover, Wnt3a induction of the posterior nervous system requires functional Meis3 in the neural plate. Using ChIP and promoter analysis, we show that Meis3 is a direct target of Wnt/β-catenin signaling. This suggests a new model for neural anteroposterior patterning, in which Wnt3a from the paraxial mesoderm induces posterior cell fates via direct activation of a crucial transcription factor in the overlying neural plate.

Original languageEnglish (US)
Pages (from-to)1531-1541
Number of pages11
JournalDevelopment
Volume137
Issue number9
DOIs
StatePublished - May 1 2010

Keywords

  • Dorsal-lateral marginal zone (DLMZ)
  • Meis3
  • Neural patterning
  • Paraxial mesoderm
  • Wnt3a
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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  • Cite this

    Elkouby, Y. M., Elias, S., Casey, E. S., Blythe, S. A., Tsabar, N., Klein, P. S., Root, H., Liu, K. J., & Frank, D. (2010). Mesodermal Wnt signaling organizes the neural plate via Meis3. Development, 137(9), 1531-1541. https://doi.org/10.1242/dev.044750