Metabolic cooperation in CHO and V79 cells following treatment with a tumor promoter

Stephen T. Warren; Larry P. Yotti; Joseph R. Moskal; Chia Cheng Chang; James E. Trosko

doi:10.1016/0014-4827(81)90250-0

Metabolic cooperation in CHO and V79 cells following treatment with a tumor promoter

Stephen T. Warren^*, Larry P. Yotti, Joseph R. Moskal, Chia Cheng Chang, James E. Trosko

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Tumor promoters are a class of chemicals which, when given to cells in vitro or to organisms that have been previously exposed to physical or chemical carcinogens, decrease the latency period for the appearance of transformed colonies or tumors. 12-O-tetradecanoyl phorbol-13-acetate (TPA), a powerful tumor promoter, has been shown to inhibit metabolic cooperation in V79 Chinese hamster cells and rat hepatocytes as well as between mouse epidermal and 3T3 cells. We report comparative studies utilizing V79 and CHO cells indicating that metabolic cooperation is inhibited by TPA in V79 cells while CHO cells show the opposite response with a slight enhancement of metabolic cooperation following promoter treatment. We speculate that these observations are the result of membrane differences between these cell lines.

Original language	English (US)
Pages (from-to)	427-430
Number of pages	4
Journal	Experimental Cell Research
Volume	131
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(81)90250-0
State	Published - Feb 1981

Funding

This work was supported by a USPHS fellowshit, (5P32-Hl07261), and NSF grant no. 78-23352 and an American Heart Association grant and Established Investigatorship Award (P. A. I.).

ASJC Scopus subject areas

Cell Biology

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10.1016/0014-4827(81)90250-0

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title = "Metabolic cooperation in CHO and V79 cells following treatment with a tumor promoter",

abstract = "Tumor promoters are a class of chemicals which, when given to cells in vitro or to organisms that have been previously exposed to physical or chemical carcinogens, decrease the latency period for the appearance of transformed colonies or tumors. 12-O-tetradecanoyl phorbol-13-acetate (TPA), a powerful tumor promoter, has been shown to inhibit metabolic cooperation in V79 Chinese hamster cells and rat hepatocytes as well as between mouse epidermal and 3T3 cells. We report comparative studies utilizing V79 and CHO cells indicating that metabolic cooperation is inhibited by TPA in V79 cells while CHO cells show the opposite response with a slight enhancement of metabolic cooperation following promoter treatment. We speculate that these observations are the result of membrane differences between these cell lines.",

author = "Warren, {Stephen T.} and Yotti, {Larry P.} and Moskal, {Joseph R.} and Chang, {Chia Cheng} and Trosko, {James E.}",

note = "Funding Information: This work was supported by a USPHS fellowshit, (5P32-Hl07261), and NSF grant no. 78-23352 and an American Heart Association grant and Established Investigatorship Award (P. A. I.).",

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AU - Warren, Stephen T.

AU - Yotti, Larry P.

AU - Moskal, Joseph R.

AU - Chang, Chia Cheng

AU - Trosko, James E.

N1 - Funding Information: This work was supported by a USPHS fellowshit, (5P32-Hl07261), and NSF grant no. 78-23352 and an American Heart Association grant and Established Investigatorship Award (P. A. I.).

PY - 1981/2

Y1 - 1981/2

N2 - Tumor promoters are a class of chemicals which, when given to cells in vitro or to organisms that have been previously exposed to physical or chemical carcinogens, decrease the latency period for the appearance of transformed colonies or tumors. 12-O-tetradecanoyl phorbol-13-acetate (TPA), a powerful tumor promoter, has been shown to inhibit metabolic cooperation in V79 Chinese hamster cells and rat hepatocytes as well as between mouse epidermal and 3T3 cells. We report comparative studies utilizing V79 and CHO cells indicating that metabolic cooperation is inhibited by TPA in V79 cells while CHO cells show the opposite response with a slight enhancement of metabolic cooperation following promoter treatment. We speculate that these observations are the result of membrane differences between these cell lines.

AB - Tumor promoters are a class of chemicals which, when given to cells in vitro or to organisms that have been previously exposed to physical or chemical carcinogens, decrease the latency period for the appearance of transformed colonies or tumors. 12-O-tetradecanoyl phorbol-13-acetate (TPA), a powerful tumor promoter, has been shown to inhibit metabolic cooperation in V79 Chinese hamster cells and rat hepatocytes as well as between mouse epidermal and 3T3 cells. We report comparative studies utilizing V79 and CHO cells indicating that metabolic cooperation is inhibited by TPA in V79 cells while CHO cells show the opposite response with a slight enhancement of metabolic cooperation following promoter treatment. We speculate that these observations are the result of membrane differences between these cell lines.

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Metabolic cooperation in CHO and V79 cells following treatment with a tumor promoter

Abstract

Funding

ASJC Scopus subject areas

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