Metabolic relationships in the cockroach, Leucophaea maderae-II. Electrophoretic analysis of dehydrogenase activity in tissue extracts

Lawrence I. Gilbert*, Erwin Goldberg

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Lactate (LDH), malate (MDH), and α-glycerophosphate (GDH) dehydrogenase were studied by polyacrylamide gel electrophoresis using various tissue extracts prepared from Leucophaea maderae. A single GDH was detected in muscle extract from various developmental stages while two forms of MDH were identified in all tissues studied. LDH-1, LDH-2, LDH-3, and LDH-5 were seen in the ovaries during all stages of the adult female's reproductive cycle. Larval ovaries and developing embryos displayed a similar pattern except for the absence of LDH-5, the slowest migrating isozyme. Sub-unit interaction and spectrophotometric experiments support this terminology. Active lactate dehydrogenase in ovaries undergoing vitellogenesis and developing embryos may be involved in supplying energy to these structures that may exist under partially anaerobic conditions.

Original languageEnglish (US)
Pages (from-to)53-63
Number of pages11
JournalJournal of Insect Physiology
Volume12
Issue number1
DOIs
StatePublished - Jan 1966

ASJC Scopus subject areas

  • Physiology
  • Insect Science

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