TY - JOUR
T1 - Metabolic signatures of gestational weight gain and postpartum weight loss in a lifestyle intervention study of overweight and obese women
AU - Lau, Chung Ho E.
AU - Taylor-Bateman, Victoria
AU - Vorkas, Panagiotis A.
AU - Graça, Gonçalo
AU - Vu, Thanh Huyen T.
AU - Hou, Lifang
AU - Chekmeneva, Elena
AU - Ebbels, Timothy M.D.
AU - Chan, Queenie
AU - Van Horn, Linda
AU - Holmes, Elaine
N1 - Funding Information:
Funding: The MOMFIT (Maternal Offspring Metabolics: Family Intervention Trial; www.clinicaltrials.gov NCT01631747) study was supported by the National Heart, Lung, and Blood Institute, National Institute of Health (US), Grant number U01HL114344.
Funding Information:
Acknowledgments: Infrastructure support for this work was provided by the NIHR Imperial Biomedical Research Centre, and in particular we thank the MRC-NIHR National Phenome Centre and Imperial Clinical Phenotyping Centre for sharing their LC-MS lipid profiling method protocols with us. Elaine Holmes is supported by the Department of Jobs, Tourism, Science and Innovation, Government of Western Australia through the Premier’s Science Fellowship Program.
PY - 2020/12
Y1 - 2020/12
N2 - Background: Overweight and obesity amongst women of reproductive age are increasingly common in developed economies and are shown to adversely affect birth outcomes and both childhood and adulthood health risks in the offspring. Metabolic profiling in conditions of overweight and obesity in pregnancy could potentially be applied to elucidate the molecular basis of the adverse effects of gestational weight gain (GWG) and postpartum weight loss (WL) on future risks for cardiovascular disease (CVD) and other chronic diseases. Methods: Biofluid samples were collected from 114 ethnically diverse pregnant women with body mass index (BMI) between 25 and 40 kg/m2 from Chicago (US), as part of a randomized lifestyle intervention trial (Maternal Offspring Metabolics: Family Intervention Trial; NCT01631747). At 15 weeks, 35 weeks of gestation, and at 1 year postpartum, the blood plasma lipidome and metabolic profile of urine samples were analyzed by liquid chromatography mass spectrometry (LC-MS) and1 H nuclear magnetic resonance spectroscopy (1 H NMR) respectively. Results: Urinary 4-deoxyerythronic acid and 4-deoxythreonic acid were found to be positively correlated to BMI. Seventeen plasma lipids were found to be associated with GWG and 16 lipids were found to be associated with WL, which included phosphatidylinositols (PI), phosphatidylcholines (PC), lysophospholipids (lyso-), sphingomyelins (SM) and ether phosphatidylcholine (PC-O). Three phospholipids found to be positively associated with GWG all contained palmitate side-chains, and amongst the 14 lipids that were negatively associated with GWG, seven were PC-O. Six of eight lipids found to be negatively associated with WL contained an 18:2 fatty acid side-chain. Conclusions: Maternal obesity was associated with characteristic urine and plasma metabolic phenotypes, and phospholipid profile was found to be associated with both GWG and postpartum WL in metabolically healthy pregnant women with overweight/obesity. Postpartum WL may be linked to the reduction in the intake of linoleic acid/conjugated linoleic acid food sources in our study population.
AB - Background: Overweight and obesity amongst women of reproductive age are increasingly common in developed economies and are shown to adversely affect birth outcomes and both childhood and adulthood health risks in the offspring. Metabolic profiling in conditions of overweight and obesity in pregnancy could potentially be applied to elucidate the molecular basis of the adverse effects of gestational weight gain (GWG) and postpartum weight loss (WL) on future risks for cardiovascular disease (CVD) and other chronic diseases. Methods: Biofluid samples were collected from 114 ethnically diverse pregnant women with body mass index (BMI) between 25 and 40 kg/m2 from Chicago (US), as part of a randomized lifestyle intervention trial (Maternal Offspring Metabolics: Family Intervention Trial; NCT01631747). At 15 weeks, 35 weeks of gestation, and at 1 year postpartum, the blood plasma lipidome and metabolic profile of urine samples were analyzed by liquid chromatography mass spectrometry (LC-MS) and1 H nuclear magnetic resonance spectroscopy (1 H NMR) respectively. Results: Urinary 4-deoxyerythronic acid and 4-deoxythreonic acid were found to be positively correlated to BMI. Seventeen plasma lipids were found to be associated with GWG and 16 lipids were found to be associated with WL, which included phosphatidylinositols (PI), phosphatidylcholines (PC), lysophospholipids (lyso-), sphingomyelins (SM) and ether phosphatidylcholine (PC-O). Three phospholipids found to be positively associated with GWG all contained palmitate side-chains, and amongst the 14 lipids that were negatively associated with GWG, seven were PC-O. Six of eight lipids found to be negatively associated with WL contained an 18:2 fatty acid side-chain. Conclusions: Maternal obesity was associated with characteristic urine and plasma metabolic phenotypes, and phospholipid profile was found to be associated with both GWG and postpartum WL in metabolically healthy pregnant women with overweight/obesity. Postpartum WL may be linked to the reduction in the intake of linoleic acid/conjugated linoleic acid food sources in our study population.
KW - Blood lipids
KW - Gestational weight gain
KW - LC-MS
KW - Lifestyle intervention
KW - Metabolic phenotyping
KW - Metabolomics
KW - NMR
UR - http://www.scopus.com/inward/record.url?scp=85097533286&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85097533286&partnerID=8YFLogxK
U2 - 10.3390/metabo10120498
DO - 10.3390/metabo10120498
M3 - Article
C2 - 33291639
AN - SCOPUS:85097533286
VL - 10
SP - 1
EP - 15
JO - Metabolites
JF - Metabolites
SN - 2218-1989
IS - 12
M1 - 498
ER -