Metabolic signatures of gestational weight gain and postpartum weight loss in a lifestyle intervention study of overweight and obese women

Chung Ho E. Lau; Victoria Taylor-Bateman; Panagiotis A. Vorkas; Gonçalo Graça; Thanh Huyen T. Vu; Lifang Hou; Elena Chekmeneva; Timothy M.D. Ebbels; Queenie Chan; Linda Van Horn; Elaine Holmes

doi:10.3390/metabo10120498

Metabolic signatures of gestational weight gain and postpartum weight loss in a lifestyle intervention study of overweight and obese women

Chung Ho E. Lau^*, Victoria Taylor-Bateman, Panagiotis A. Vorkas, Gonçalo Graça, Thanh Huyen T. Vu, Lifang Hou, Elena Chekmeneva, Timothy M.D. Ebbels, Queenie Chan, Linda Van Horn, Elaine Holmes^*

^*Corresponding author for this work

Preventive Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Background: Overweight and obesity amongst women of reproductive age are increasingly common in developed economies and are shown to adversely affect birth outcomes and both childhood and adulthood health risks in the offspring. Metabolic profiling in conditions of overweight and obesity in pregnancy could potentially be applied to elucidate the molecular basis of the adverse effects of gestational weight gain (GWG) and postpartum weight loss (WL) on future risks for cardiovascular disease (CVD) and other chronic diseases. Methods: Biofluid samples were collected from 114 ethnically diverse pregnant women with body mass index (BMI) between 25 and 40 kg/m² from Chicago (US), as part of a randomized lifestyle intervention trial (Maternal Offspring Metabolics: Family Intervention Trial; NCT01631747). At 15 weeks, 35 weeks of gestation, and at 1 year postpartum, the blood plasma lipidome and metabolic profile of urine samples were analyzed by liquid chromatography mass spectrometry (LC-MS) and¹ H nuclear magnetic resonance spectroscopy (¹ H NMR) respectively. Results: Urinary 4-deoxyerythronic acid and 4-deoxythreonic acid were found to be positively correlated to BMI. Seventeen plasma lipids were found to be associated with GWG and 16 lipids were found to be associated with WL, which included phosphatidylinositols (PI), phosphatidylcholines (PC), lysophospholipids (lyso-), sphingomyelins (SM) and ether phosphatidylcholine (PC-O). Three phospholipids found to be positively associated with GWG all contained palmitate side-chains, and amongst the 14 lipids that were negatively associated with GWG, seven were PC-O. Six of eight lipids found to be negatively associated with WL contained an 18:2 fatty acid side-chain. Conclusions: Maternal obesity was associated with characteristic urine and plasma metabolic phenotypes, and phospholipid profile was found to be associated with both GWG and postpartum WL in metabolically healthy pregnant women with overweight/obesity. Postpartum WL may be linked to the reduction in the intake of linoleic acid/conjugated linoleic acid food sources in our study population.

Original language	English (US)
Article number	498
Pages (from-to)	1-15
Number of pages	15
Journal	Metabolites
Volume	10
Issue number	12
DOIs	https://doi.org/10.3390/metabo10120498
State	Published - Dec 2020

Funding

Funding: The MOMFIT (Maternal Offspring Metabolics: Family Intervention Trial; www.clinicaltrials.gov NCT01631747) study was supported by the National Heart, Lung, and Blood Institute, National Institute of Health (US), Grant number U01HL114344. Acknowledgments: Infrastructure support for this work was provided by the NIHR Imperial Biomedical Research Centre, and in particular we thank the MRC-NIHR National Phenome Centre and Imperial Clinical Phenotyping Centre for sharing their LC-MS lipid profiling method protocols with us. Elaine Holmes is supported by the Department of Jobs, Tourism, Science and Innovation, Government of Western Australia through the Premier’s Science Fellowship Program.

Keywords

Blood lipids
Gestational weight gain
LC-MS
Lifestyle intervention
Metabolic phenotyping
Metabolomics
NMR

ASJC Scopus subject areas

Molecular Biology
Biochemistry
Endocrinology, Diabetes and Metabolism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/metabo10120498

Cite this

Lau, C. H. E., Taylor-Bateman, V., Vorkas, P. A., Graça, G., Vu, T. H. T., Hou, L., Chekmeneva, E., Ebbels, T. M. D., Chan, Q., Van Horn, L., & Holmes, E. (2020). Metabolic signatures of gestational weight gain and postpartum weight loss in a lifestyle intervention study of overweight and obese women. Metabolites, 10(12), 1-15. Article 498. https://doi.org/10.3390/metabo10120498

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title = "Metabolic signatures of gestational weight gain and postpartum weight loss in a lifestyle intervention study of overweight and obese women",

abstract = "Background: Overweight and obesity amongst women of reproductive age are increasingly common in developed economies and are shown to adversely affect birth outcomes and both childhood and adulthood health risks in the offspring. Metabolic profiling in conditions of overweight and obesity in pregnancy could potentially be applied to elucidate the molecular basis of the adverse effects of gestational weight gain (GWG) and postpartum weight loss (WL) on future risks for cardiovascular disease (CVD) and other chronic diseases. Methods: Biofluid samples were collected from 114 ethnically diverse pregnant women with body mass index (BMI) between 25 and 40 kg/m2 from Chicago (US), as part of a randomized lifestyle intervention trial (Maternal Offspring Metabolics: Family Intervention Trial; NCT01631747). At 15 weeks, 35 weeks of gestation, and at 1 year postpartum, the blood plasma lipidome and metabolic profile of urine samples were analyzed by liquid chromatography mass spectrometry (LC-MS) and1 H nuclear magnetic resonance spectroscopy (1 H NMR) respectively. Results: Urinary 4-deoxyerythronic acid and 4-deoxythreonic acid were found to be positively correlated to BMI. Seventeen plasma lipids were found to be associated with GWG and 16 lipids were found to be associated with WL, which included phosphatidylinositols (PI), phosphatidylcholines (PC), lysophospholipids (lyso-), sphingomyelins (SM) and ether phosphatidylcholine (PC-O). Three phospholipids found to be positively associated with GWG all contained palmitate side-chains, and amongst the 14 lipids that were negatively associated with GWG, seven were PC-O. Six of eight lipids found to be negatively associated with WL contained an 18:2 fatty acid side-chain. Conclusions: Maternal obesity was associated with characteristic urine and plasma metabolic phenotypes, and phospholipid profile was found to be associated with both GWG and postpartum WL in metabolically healthy pregnant women with overweight/obesity. Postpartum WL may be linked to the reduction in the intake of linoleic acid/conjugated linoleic acid food sources in our study population.",

keywords = "Blood lipids, Gestational weight gain, LC-MS, Lifestyle intervention, Metabolic phenotyping, Metabolomics, NMR",

author = "Lau, {Chung Ho E.} and Victoria Taylor-Bateman and Vorkas, {Panagiotis A.} and Gon{\c c}alo Gra{\c c}a and Vu, {Thanh Huyen T.} and Lifang Hou and Elena Chekmeneva and Ebbels, {Timothy M.D.} and Queenie Chan and {Van Horn}, Linda and Elaine Holmes",

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year = "2020",

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doi = "10.3390/metabo10120498",

language = "English (US)",

volume = "10",

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T1 - Metabolic signatures of gestational weight gain and postpartum weight loss in a lifestyle intervention study of overweight and obese women

AU - Lau, Chung Ho E.

AU - Taylor-Bateman, Victoria

AU - Vorkas, Panagiotis A.

AU - Graça, Gonçalo

AU - Vu, Thanh Huyen T.

AU - Hou, Lifang

AU - Chekmeneva, Elena

AU - Ebbels, Timothy M.D.

AU - Chan, Queenie

AU - Van Horn, Linda

AU - Holmes, Elaine

PY - 2020/12

Y1 - 2020/12

N2 - Background: Overweight and obesity amongst women of reproductive age are increasingly common in developed economies and are shown to adversely affect birth outcomes and both childhood and adulthood health risks in the offspring. Metabolic profiling in conditions of overweight and obesity in pregnancy could potentially be applied to elucidate the molecular basis of the adverse effects of gestational weight gain (GWG) and postpartum weight loss (WL) on future risks for cardiovascular disease (CVD) and other chronic diseases. Methods: Biofluid samples were collected from 114 ethnically diverse pregnant women with body mass index (BMI) between 25 and 40 kg/m2 from Chicago (US), as part of a randomized lifestyle intervention trial (Maternal Offspring Metabolics: Family Intervention Trial; NCT01631747). At 15 weeks, 35 weeks of gestation, and at 1 year postpartum, the blood plasma lipidome and metabolic profile of urine samples were analyzed by liquid chromatography mass spectrometry (LC-MS) and1 H nuclear magnetic resonance spectroscopy (1 H NMR) respectively. Results: Urinary 4-deoxyerythronic acid and 4-deoxythreonic acid were found to be positively correlated to BMI. Seventeen plasma lipids were found to be associated with GWG and 16 lipids were found to be associated with WL, which included phosphatidylinositols (PI), phosphatidylcholines (PC), lysophospholipids (lyso-), sphingomyelins (SM) and ether phosphatidylcholine (PC-O). Three phospholipids found to be positively associated with GWG all contained palmitate side-chains, and amongst the 14 lipids that were negatively associated with GWG, seven were PC-O. Six of eight lipids found to be negatively associated with WL contained an 18:2 fatty acid side-chain. Conclusions: Maternal obesity was associated with characteristic urine and plasma metabolic phenotypes, and phospholipid profile was found to be associated with both GWG and postpartum WL in metabolically healthy pregnant women with overweight/obesity. Postpartum WL may be linked to the reduction in the intake of linoleic acid/conjugated linoleic acid food sources in our study population.

AB - Background: Overweight and obesity amongst women of reproductive age are increasingly common in developed economies and are shown to adversely affect birth outcomes and both childhood and adulthood health risks in the offspring. Metabolic profiling in conditions of overweight and obesity in pregnancy could potentially be applied to elucidate the molecular basis of the adverse effects of gestational weight gain (GWG) and postpartum weight loss (WL) on future risks for cardiovascular disease (CVD) and other chronic diseases. Methods: Biofluid samples were collected from 114 ethnically diverse pregnant women with body mass index (BMI) between 25 and 40 kg/m2 from Chicago (US), as part of a randomized lifestyle intervention trial (Maternal Offspring Metabolics: Family Intervention Trial; NCT01631747). At 15 weeks, 35 weeks of gestation, and at 1 year postpartum, the blood plasma lipidome and metabolic profile of urine samples were analyzed by liquid chromatography mass spectrometry (LC-MS) and1 H nuclear magnetic resonance spectroscopy (1 H NMR) respectively. Results: Urinary 4-deoxyerythronic acid and 4-deoxythreonic acid were found to be positively correlated to BMI. Seventeen plasma lipids were found to be associated with GWG and 16 lipids were found to be associated with WL, which included phosphatidylinositols (PI), phosphatidylcholines (PC), lysophospholipids (lyso-), sphingomyelins (SM) and ether phosphatidylcholine (PC-O). Three phospholipids found to be positively associated with GWG all contained palmitate side-chains, and amongst the 14 lipids that were negatively associated with GWG, seven were PC-O. Six of eight lipids found to be negatively associated with WL contained an 18:2 fatty acid side-chain. Conclusions: Maternal obesity was associated with characteristic urine and plasma metabolic phenotypes, and phospholipid profile was found to be associated with both GWG and postpartum WL in metabolically healthy pregnant women with overweight/obesity. Postpartum WL may be linked to the reduction in the intake of linoleic acid/conjugated linoleic acid food sources in our study population.

KW - Blood lipids

KW - Gestational weight gain

KW - LC-MS

KW - Lifestyle intervention

KW - Metabolic phenotyping

KW - Metabolomics

KW - NMR

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Metabolic signatures of gestational weight gain and postpartum weight loss in a lifestyle intervention study of overweight and obese women

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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