Metabotropic glutamate receptor 5/homer interactions underlie stress effects on fear

Natalie C. Tronson, Yomayra F. Guzman, Anita L. Guedea, Kyu Hwan Huh, Can Gao, Martin K. Schwarz, Jelena Radulovic*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

61 Scopus citations


Background Glutamatergic transmission is one of the main components of the stress response; nevertheless, its role in the emotional stress sequelae is not known. Here, we investigated whether interactions between group I metabotropic glutamate receptors (metabotropic glutamate receptor 1 and metabotropic glutamate receptor 5 [mGluR5]) and Homer proteins mediate the delayed and persistent enhancement of fear induced by acute stress. Methods Antagonists and inverse agonists of metabotropic glutamate receptor 1 and mGluR5 were injected into the hippocampus after immobilization stress and before contextual fear conditioning. Metabotropic glutamate receptor 5 was displaced from constitutive Homer scaffolds by viral transfection of Homer1a or injection of Tat decoy peptides. The effects of these manipulations on stress-enhanced fear were determined. Results We show that stress induces interactions between hippocampal mGluR5 and Homer1a; causes a sustained, ligand-independent mGluR5 activity; and enhances contextual fear. Consistent with this mechanism, enhancement of fear was abolished by delayed poststress application of inverse agonists, but not antagonists, of mGluR5. The effect of stress was mimicked by virally transfected Homer1a or injection of Tat-metabotropic glutamate receptor C-tail decoy peptides into the hippocampus. Conclusions Constitutive activation of mGluR5 is identified as a principal hippocampal mechanism underlying the delayed stress effects on emotion and memory. Inverse agonists, but not antagonists, of mGluR5 are therefore proposed as a preventive treatment option for acute and posttraumatic stress disorders.

Original languageEnglish (US)
Pages (from-to)1007-1015
Number of pages9
JournalBiological psychiatry
Issue number11
StatePublished - Dec 1 2010


  • Conditioned fear
  • Constitutive activity
  • Homer
  • Metabotropic glutamate receptor 5
  • Posttraumatic stress disorder
  • Stress

ASJC Scopus subject areas

  • Biological Psychiatry


Dive into the research topics of 'Metabotropic glutamate receptor 5/homer interactions underlie stress effects on fear'. Together they form a unique fingerprint.

Cite this