Method to identify and minimize artifacts induced by fluorescent impurities in single-molecule localization microscopy

Janel L. Davis, Biqin Dong, Cheng Sun, Hao F. Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The existence of fluorescent impurities has been a long-standing obstacle in single-molecule imaging, which results in sample misidentification and higher localization uncertainty. Spectroscopic single-molecule localization microscopy can record the full fluorescent spectrum of every stochastic single-molecule emission event. This capability allows us to quantify the spatial and spectral characteristics of fluorescent impurities introduced by sample preparation steps, based on which we developed a method to effectively separate fluorescent impurities from target molecules.

Original languageEnglish (US)
Article number106501
JournalJournal of Biomedical Optics
Volume23
Issue number10
DOIs
StatePublished - Oct 1 2018

Keywords

  • fluorescence spectral imaging
  • fluorescent impurities
  • single-molecule localization microscopy
  • single-molecule spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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