Methods for characterization of alternative RNA splicing

Samuel E. Harvey, Chonghui Cheng

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations

Abstract

Quantification of alternative splicing to detect the abundance of differentially spliced isoforms of a gene in total RNA can be accomplished via RT-PCR using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of particular splice isoforms. We also describe analysis of alternative splicing using a splicing “minigene” in mammalian cell tissue culture to facilitate investigation of the regulation of alternative splicing of a particular exon of interest.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages229-241
Number of pages13
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1402
ISSN (Print)1064-3745

Keywords

  • Alternative splicing
  • Minigene
  • RNA
  • RT-PCR
  • Splicing
  • Splicing factors
  • Variable exon
  • regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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