Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments

Karen M. Ridge*, Dale Shumaker, Amélie Robert, Caroline Hookway, Vladimir I. Gelfand, Paul A. Janmey, Jason Lowery, Ming Guo, David A. Weitz, Edward Kuczmarski, Robert D. Goldman

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations

Abstract

The type III intermediate filament protein vimentin was once thought to function mainly as a static structural protein in the cytoskeleton of cells of mesenchymal origin. Now, however, vimentin is known to form a dynamic, flexible network that plays an important role in a number of signaling pathways. Here, we describe various methods that have been developed to investigate the cellular functions of the vimentin protein and intermediate filament network, including chemical disruption, photoactivation and photoconversion, biolayer interferometry, soluble bead binding assay, three-dimensional substrate experiments, collagen gel contraction, optical-tweezer active microrheology, and force spectrum microscopy. Using these techniques, the contributions of vimentin to essential cellular processes can be probed in ever further detail.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc
Pages389-426
Number of pages38
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Enzymology
Volume568
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Biolayer interferometry
  • Collagen gel contraction
  • Force spectrum microscopy
  • Gigaxonin
  • Optical tweezers
  • Photoactivation
  • Photoconversion
  • Three-dimensional substrate
  • Vimentin
  • Withaferin A

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments'. Together they form a unique fingerprint.

  • Cite this

    Ridge, K. M., Shumaker, D., Robert, A., Hookway, C., Gelfand, V. I., Janmey, P. A., Lowery, J., Guo, M., Weitz, D. A., Kuczmarski, E., & Goldman, R. D. (2016). Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments. In Methods in Enzymology (pp. 389-426). (Methods in Enzymology; Vol. 568). Academic Press Inc. https://doi.org/10.1016/bs.mie.2015.09.036