Abstract
The type III intermediate filament protein vimentin was once thought to function mainly as a static structural protein in the cytoskeleton of cells of mesenchymal origin. Now, however, vimentin is known to form a dynamic, flexible network that plays an important role in a number of signaling pathways. Here, we describe various methods that have been developed to investigate the cellular functions of the vimentin protein and intermediate filament network, including chemical disruption, photoactivation and photoconversion, biolayer interferometry, soluble bead binding assay, three-dimensional substrate experiments, collagen gel contraction, optical-tweezer active microrheology, and force spectrum microscopy. Using these techniques, the contributions of vimentin to essential cellular processes can be probed in ever further detail.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Enzymology |
| Publisher | Academic Press Inc |
| Pages | 389-426 |
| Number of pages | 38 |
| DOIs | |
| State | Published - Jan 1 2016 |
Publication series
| Name | Methods in Enzymology |
|---|---|
| Volume | 568 |
| ISSN (Print) | 0076-6879 |
| ISSN (Electronic) | 1557-7988 |
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Keywords
- Biolayer interferometry
- Collagen gel contraction
- Force spectrum microscopy
- Gigaxonin
- Optical tweezers
- Photoactivation
- Photoconversion
- Three-dimensional substrate
- Vimentin
- Withaferin A
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
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Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments. / Ridge, Karen M.; Shumaker, Dale; Robert, Amélie; Hookway, Caroline; Gelfand, Vladimir I.; Janmey, Paul A.; Lowery, Jason; Guo, Ming; Weitz, David A.; Kuczmarski, Edward; Goldman, Robert D.
Methods in Enzymology. Academic Press Inc, 2016. p. 389-426 (Methods in Enzymology; Vol. 568).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments
AU - Ridge, Karen M.
AU - Shumaker, Dale
AU - Robert, Amélie
AU - Hookway, Caroline
AU - Gelfand, Vladimir I.
AU - Janmey, Paul A.
AU - Lowery, Jason
AU - Guo, Ming
AU - Weitz, David A.
AU - Kuczmarski, Edward
AU - Goldman, Robert D.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - The type III intermediate filament protein vimentin was once thought to function mainly as a static structural protein in the cytoskeleton of cells of mesenchymal origin. Now, however, vimentin is known to form a dynamic, flexible network that plays an important role in a number of signaling pathways. Here, we describe various methods that have been developed to investigate the cellular functions of the vimentin protein and intermediate filament network, including chemical disruption, photoactivation and photoconversion, biolayer interferometry, soluble bead binding assay, three-dimensional substrate experiments, collagen gel contraction, optical-tweezer active microrheology, and force spectrum microscopy. Using these techniques, the contributions of vimentin to essential cellular processes can be probed in ever further detail.
AB - The type III intermediate filament protein vimentin was once thought to function mainly as a static structural protein in the cytoskeleton of cells of mesenchymal origin. Now, however, vimentin is known to form a dynamic, flexible network that plays an important role in a number of signaling pathways. Here, we describe various methods that have been developed to investigate the cellular functions of the vimentin protein and intermediate filament network, including chemical disruption, photoactivation and photoconversion, biolayer interferometry, soluble bead binding assay, three-dimensional substrate experiments, collagen gel contraction, optical-tweezer active microrheology, and force spectrum microscopy. Using these techniques, the contributions of vimentin to essential cellular processes can be probed in ever further detail.
KW - Biolayer interferometry
KW - Collagen gel contraction
KW - Force spectrum microscopy
KW - Gigaxonin
KW - Optical tweezers
KW - Photoactivation
KW - Photoconversion
KW - Three-dimensional substrate
KW - Vimentin
KW - Withaferin A
UR - http://www.scopus.com/inward/record.url?scp=84958063731&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84958063731&partnerID=8YFLogxK
U2 - 10.1016/bs.mie.2015.09.036
DO - 10.1016/bs.mie.2015.09.036
M3 - Chapter
C2 - 26795478
AN - SCOPUS:84958063731
T3 - Methods in Enzymology
SP - 389
EP - 426
BT - Methods in Enzymology
PB - Academic Press Inc
ER -