@inbook{d79abfcc1cd34f9ca213d336df8e5f69,
title = "Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments",
abstract = "The type III intermediate filament protein vimentin was once thought to function mainly as a static structural protein in the cytoskeleton of cells of mesenchymal origin. Now, however, vimentin is known to form a dynamic, flexible network that plays an important role in a number of signaling pathways. Here, we describe various methods that have been developed to investigate the cellular functions of the vimentin protein and intermediate filament network, including chemical disruption, photoactivation and photoconversion, biolayer interferometry, soluble bead binding assay, three-dimensional substrate experiments, collagen gel contraction, optical-tweezer active microrheology, and force spectrum microscopy. Using these techniques, the contributions of vimentin to essential cellular processes can be probed in ever further detail.",
keywords = "Biolayer interferometry, Collagen gel contraction, Force spectrum microscopy, Gigaxonin, Optical tweezers, Photoactivation, Photoconversion, Three-dimensional substrate, Vimentin, Withaferin A",
author = "Ridge, {Karen M.} and Dale Shumaker and Am{\'e}lie Robert and Caroline Hookway and Gelfand, {Vladimir I.} and Janmey, {Paul A.} and Jason Lowery and Ming Guo and Weitz, {David A.} and Edward Kuczmarski and Goldman, {Robert D.}",
note = "Funding Information: Funding: R.D.G. is supported by grants from the National Institute of General Medical Sciences (P01GM09697), National Institutes of Health and the Hannah's Hope Fund. V.I.G. is supported by grants from the National Institute of General Medical Sciences (P01GM09697 and GM052111), National Institutes of Health. K.M.R. is supported by the National Heart, Lung, and Blood Institute (HL71643; HL124664), Department of Veterans Affairs (MERIT Award). P.A.J. is supported by grants from the National Institutes of health (GM096971 and EB017753). D.A.W. is supported by the NIH (PO1GM096971), the Harvard Materials Research Science and Engineering Center (DMR-0820484). Publisher Copyright: Copyright {\textcopyright} 2016 Elsevier Inc. All rights reserved.",
year = "2016",
doi = "10.1016/bs.mie.2015.09.036",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc",
pages = "389--426",
booktitle = "Methods in Enzymology",
}