Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments

Karen M. Ridge*, Dale Shumaker, Amélie Robert, Caroline Hookway, Vladimir I. Gelfand, Paul A. Janmey, Jason Lowery, Ming Guo, David A. Weitz, Edward Kuczmarski, Robert D. Goldman

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

The type III intermediate filament protein vimentin was once thought to function mainly as a static structural protein in the cytoskeleton of cells of mesenchymal origin. Now, however, vimentin is known to form a dynamic, flexible network that plays an important role in a number of signaling pathways. Here, we describe various methods that have been developed to investigate the cellular functions of the vimentin protein and intermediate filament network, including chemical disruption, photoactivation and photoconversion, biolayer interferometry, soluble bead binding assay, three-dimensional substrate experiments, collagen gel contraction, optical-tweezer active microrheology, and force spectrum microscopy. Using these techniques, the contributions of vimentin to essential cellular processes can be probed in ever further detail.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages389-426
Number of pages38
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Enzymology
Volume568
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Fingerprint

Intermediate Filaments
Vimentin
Intermediate Filament Proteins
Optical Tweezers
Interferometry
Optical tweezers
Atomic Force Microscopy
Cytoskeleton
Assays
Microscopic examination
Collagen
Gels
Substrates
Proteins
Experiments

Keywords

  • Biolayer interferometry
  • Collagen gel contraction
  • Force spectrum microscopy
  • Gigaxonin
  • Optical tweezers
  • Photoactivation
  • Photoconversion
  • Three-dimensional substrate
  • Vimentin
  • Withaferin A

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Ridge, K. M., Shumaker, D., Robert, A., Hookway, C., Gelfand, V. I., Janmey, P. A., ... Goldman, R. D. (2016). Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments. In Methods in Enzymology (pp. 389-426). (Methods in Enzymology; Vol. 568). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2015.09.036
Ridge, Karen M. ; Shumaker, Dale ; Robert, Amélie ; Hookway, Caroline ; Gelfand, Vladimir I. ; Janmey, Paul A. ; Lowery, Jason ; Guo, Ming ; Weitz, David A. ; Kuczmarski, Edward ; Goldman, Robert D. / Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments. Methods in Enzymology. Academic Press Inc., 2016. pp. 389-426 (Methods in Enzymology).
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Ridge, KM, Shumaker, D, Robert, A, Hookway, C, Gelfand, VI, Janmey, PA, Lowery, J, Guo, M, Weitz, DA, Kuczmarski, E & Goldman, RD 2016, Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments. in Methods in Enzymology. Methods in Enzymology, vol. 568, Academic Press Inc., pp. 389-426. https://doi.org/10.1016/bs.mie.2015.09.036

Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments. / Ridge, Karen M.; Shumaker, Dale; Robert, Amélie; Hookway, Caroline; Gelfand, Vladimir I.; Janmey, Paul A.; Lowery, Jason; Guo, Ming; Weitz, David A.; Kuczmarski, Edward; Goldman, Robert D.

Methods in Enzymology. Academic Press Inc., 2016. p. 389-426 (Methods in Enzymology; Vol. 568).

Research output: Chapter in Book/Report/Conference proceedingChapter

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AU - Robert, Amélie

AU - Hookway, Caroline

AU - Gelfand, Vladimir I.

AU - Janmey, Paul A.

AU - Lowery, Jason

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AU - Kuczmarski, Edward

AU - Goldman, Robert D.

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KW - Gigaxonin

KW - Optical tweezers

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Ridge KM, Shumaker D, Robert A, Hookway C, Gelfand VI, Janmey PA et al. Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments. In Methods in Enzymology. Academic Press Inc. 2016. p. 389-426. (Methods in Enzymology). https://doi.org/10.1016/bs.mie.2015.09.036