Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts

Anabela S. Ramalho; Sebastian Beck; Carlos M. Farinha; Luka A. Clarke; Ghanshyam D. Heda; Bernhard Steiner; Javier Sanz; Sabina Gallati; Margarida D. Amaral; Ann Harris; Maria Tzetis

doi:10.1016/j.jcf.2004.05.004

Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts

Anabela S. Ramalho, Sebastian Beck, Carlos M. Farinha, Luka A. Clarke, Ghanshyam D. Heda, Bernhard Steiner, Javier Sanz, Sabina Gallati, Margarida D. Amaral, Ann Harris, Maria Tzetis^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The scope of this article is to outline some of the basic methods for good quality RNA preparation from mammalian tissues and cells (including epithelial cells). Additionally, we give an outline of common techniques of measuring CFTR gene expression such as quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease protection assay (RPA). These methods are designed to detect low abundance transcripts, which apply to CFTR mRNA in most cell types and tissues.

Original language	English (US)
Pages (from-to)	11-15
Number of pages	5
Journal	Journal of Cystic Fibrosis
Volume	3
Issue number	SUPPL. 2
DOIs	https://doi.org/10.1016/j.jcf.2004.05.004
State	Published - Aug 2004

Keywords

CFTR
Cystic fibrosis
Epithelial cells
RT-PCR
Ribonuclease protection assay
mRNA

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine
Pediatrics, Perinatology, and Child Health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jcf.2004.05.004

Cite this

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title = "Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts",

abstract = "The scope of this article is to outline some of the basic methods for good quality RNA preparation from mammalian tissues and cells (including epithelial cells). Additionally, we give an outline of common techniques of measuring CFTR gene expression such as quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease protection assay (RPA). These methods are designed to detect low abundance transcripts, which apply to CFTR mRNA in most cell types and tissues.",

keywords = "CFTR, Cystic fibrosis, Epithelial cells, RT-PCR, Ribonuclease protection assay, mRNA",

author = "Ramalho, {Anabela S.} and Sebastian Beck and Farinha, {Carlos M.} and Clarke, {Luka A.} and Heda, {Ghanshyam D.} and Bernhard Steiner and Javier Sanz and Sabina Gallati and Amaral, {Margarida D.} and Ann Harris and Maria Tzetis",

year = "2004",

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doi = "10.1016/j.jcf.2004.05.004",

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T1 - Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts

AU - Ramalho, Anabela S.

AU - Beck, Sebastian

AU - Farinha, Carlos M.

AU - Clarke, Luka A.

AU - Heda, Ghanshyam D.

AU - Steiner, Bernhard

AU - Sanz, Javier

AU - Gallati, Sabina

AU - Amaral, Margarida D.

AU - Harris, Ann

AU - Tzetis, Maria

PY - 2004/8

Y1 - 2004/8

N2 - The scope of this article is to outline some of the basic methods for good quality RNA preparation from mammalian tissues and cells (including epithelial cells). Additionally, we give an outline of common techniques of measuring CFTR gene expression such as quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease protection assay (RPA). These methods are designed to detect low abundance transcripts, which apply to CFTR mRNA in most cell types and tissues.

AB - The scope of this article is to outline some of the basic methods for good quality RNA preparation from mammalian tissues and cells (including epithelial cells). Additionally, we give an outline of common techniques of measuring CFTR gene expression such as quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease protection assay (RPA). These methods are designed to detect low abundance transcripts, which apply to CFTR mRNA in most cell types and tissues.

KW - CFTR

KW - Cystic fibrosis

KW - Epithelial cells

KW - RT-PCR

KW - Ribonuclease protection assay

KW - mRNA

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AN - SCOPUS:19944409297

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VL - 3

SP - 11

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JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

IS - SUPPL. 2

ER -

Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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