Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts

Anabela S. Ramalho, Sebastian Beck, Carlos M. Farinha, Luka A. Clarke, Ghanshyam D. Heda, Bernhard Steiner, Javier Sanz, Sabina Gallati, Margarida D. Amaral, Ann Harris, Maria Tzetis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


The scope of this article is to outline some of the basic methods for good quality RNA preparation from mammalian tissues and cells (including epithelial cells). Additionally, we give an outline of common techniques of measuring CFTR gene expression such as quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease protection assay (RPA). These methods are designed to detect low abundance transcripts, which apply to CFTR mRNA in most cell types and tissues.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalJournal of Cystic Fibrosis
Issue numberSUPPL. 2
StatePublished - Aug 2004


  • CFTR
  • Cystic fibrosis
  • Epithelial cells
  • RT-PCR
  • Ribonuclease protection assay
  • mRNA

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pulmonary and Respiratory Medicine


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