Methods related to studying tau fragmentation

Adriana Ferreira*, Sana Afreen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


Several tau posttranslational modifications have been implicated in neuronal degeneration. Among them, tau fragmentation has been identified not only in brain samples obtained from Alzheimer's disease (AD) and related disorder subjects but also in AD culture and animal models. Some of these tau fragments have not been extensively studied. In contrast, data obtained recently showed that tau fragmentation mediated by enhanced or abnormal calpain, caspase 2, caspase 3, and asparagine endopeptidase activity results in the formation of toxic fragments. These cleaved tau forms induce neuronal death, synapse loss, and/or behavioral deficits. Here, we described protease activity assays and methods to study the effects of tau fragments on neuronal viability.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc
Number of pages14
StatePublished - 2017

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X


  • Calpain
  • Caspases
  • Cell viability assays
  • Endopeptidases
  • Neuronal nucleofection
  • Protease activity assays
  • Tau cleavage

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Methods related to studying tau fragmentation'. Together they form a unique fingerprint.

Cite this