Methods related to studying tau fragmentation

Adriana Ferreira*, Sana Afreen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Several tau posttranslational modifications have been implicated in neuronal degeneration. Among them, tau fragmentation has been identified not only in brain samples obtained from Alzheimer's disease (AD) and related disorder subjects but also in AD culture and animal models. Some of these tau fragments have not been extensively studied. In contrast, data obtained recently showed that tau fragmentation mediated by enhanced or abnormal calpain, caspase 2, caspase 3, and asparagine endopeptidase activity results in the formation of toxic fragments. These cleaved tau forms induce neuronal death, synapse loss, and/or behavioral deficits. Here, we described protease activity assays and methods to study the effects of tau fragments on neuronal viability.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc
Pages245-258
Number of pages14
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Cell Biology
Volume141
ISSN (Print)0091-679X

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Keywords

  • Calpain
  • Caspases
  • Cell viability assays
  • Endopeptidases
  • Neuronal nucleofection
  • Protease activity assays
  • Tau cleavage

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ferreira, A., & Afreen, S. (2017). Methods related to studying tau fragmentation. In Methods in Cell Biology (pp. 245-258). (Methods in Cell Biology; Vol. 141). Academic Press Inc. https://doi.org/10.1016/bs.mcb.2017.06.004