TY - JOUR
T1 - Methylation inhibits the interaction of dna binding proteins with a potential C-ABL intron regulatory element
AU - Hengst-Zhang, Jamie A.
AU - Weitzman, Sigmund A.
N1 - Funding Information:
The authors wish to thank Gavin Somersel and Eric Silberman for technical assistance. We thank Dr. Richard C. Scarpulla of Northwestern University for supplying the plasmid pRC4CATBA/-66BA containing the rat somatic cytochrome C promoter and CAT sequences and for helpful advice. Finally, we also thank Pat Turk, Steve Satek and Dr. Ji Zhang for helpful discussion and Marie Lee for help in manuscript preparation. This work was supported by USPHS grant #R0147549 to Dr. Sigmund Weitzman.
PY - 1992/8/14
Y1 - 1992/8/14
N2 - We have identified DNA binding proteins which interact with a sequence found in an intron of the tyrosine kinase coding portion of the murine c-abl gene. Several specific DNA:protein complexes were observed. Those complexes of approximate molecular weights 64 and 66kDa were detected when an Msp I site (CCGG) within the sequence was unmethylated, but were not observed when that site was methylated. Insertion of the intron sequence 5′ to the rat somatic cytochrome C promoter and chloramphenicol acetyl transferase (CAT) sequences resulted in at least four-fold stimulation of CAT activity. These data suggest a potential role for the intron sequence in the regulation of gene expression.
AB - We have identified DNA binding proteins which interact with a sequence found in an intron of the tyrosine kinase coding portion of the murine c-abl gene. Several specific DNA:protein complexes were observed. Those complexes of approximate molecular weights 64 and 66kDa were detected when an Msp I site (CCGG) within the sequence was unmethylated, but were not observed when that site was methylated. Insertion of the intron sequence 5′ to the rat somatic cytochrome C promoter and chloramphenicol acetyl transferase (CAT) sequences resulted in at least four-fold stimulation of CAT activity. These data suggest a potential role for the intron sequence in the regulation of gene expression.
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U2 - 10.1016/S0006-291X(05)81578-5
DO - 10.1016/S0006-291X(05)81578-5
M3 - Article
C2 - 1510678
AN - SCOPUS:0026969124
SN - 0006-291X
VL - 186
SP - 1515
EP - 1521
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -