Methylation inhibits the interaction of dna binding proteins with a potential C-ABL intron regulatory element

Jamie A. Hengst-Zhang, Sigmund A. Weitzman*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We have identified DNA binding proteins which interact with a sequence found in an intron of the tyrosine kinase coding portion of the murine c-abl gene. Several specific DNA:protein complexes were observed. Those complexes of approximate molecular weights 64 and 66kDa were detected when an Msp I site (CCGG) within the sequence was unmethylated, but were not observed when that site was methylated. Insertion of the intron sequence 5′ to the rat somatic cytochrome C promoter and chloramphenicol acetyl transferase (CAT) sequences resulted in at least four-fold stimulation of CAT activity. These data suggest a potential role for the intron sequence in the regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)1515-1521
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number3
DOIs
StatePublished - Aug 14 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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