Abstract
Background and Objectives: Detection of pancreatic cancer by blood-based test may improve outcomes. We sought to establish the feasibility of a blood-based detection of pancreatic cancer through multiplexed array-mediated analysis of DNA methylation. Methods: Methylation was assessed in each plasma sample using a panel of 56 frequently methylated genes. Methylation profiles in patients with ductal cell adenocarcinoma of the pancreas (n = 30) and healthy gender and age-matched controls (n = 30) were compared. Methylation was determined as described previously; a composite biomarker was developed for classification of cancer and normal samples. Sensitivity and specificity of the biomarker were estimated using 25 rounds of fivefold cross-validation. Results: Five promoters were consistently selected for the classifier during cross-validation and comprised the final composite biomarker Fivefold cross-validation results indicate 76% sensitivity and 59% specificity of the biomarker, which included promoters of CCND2, SOCS1, THBS1, PLAU, and VHL. Conclusion: Differential methylation profiling of plasma DNA can detect ductal adenocarcinoma of the pancreas with significant accuracy and should be explored further. While additional improvement of biomarkers is necessary, the blood-based biomarker may be already useful as a first-line detection tool.
Original language | English (US) |
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Pages (from-to) | 119-122 |
Number of pages | 4 |
Journal | Journal of surgical oncology |
Volume | 99 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1 2009 |
Keywords
- Composite biomarker
- DNA methylation
- Detection
- Pancreatic cancer
ASJC Scopus subject areas
- Oncology
- Surgery