Microanalysis of glycosaminoglycans

Moira Breen*, Paul A. Knepper, Hyman G. Weinstein, Lawrence J. Blacik, Dianne G. Lewandowski, Bebe M. Baltrus

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

A sensitive and versatile method for the qualitative and quantitative determination of glycosaminoglycans (GAGs) is described. An enriched GAG fraction was subjected to nuclease enzyme treatment and to an appropriate sequence of GAG degrading enzymes-Streptomyces hyaluronidase, chondroitinase AC and ABC, and endo-β-d-galactosidase-and nitrous acid treatment. To determine the result of each degradative procedure, the remaining GAG polymers were subjected to cellulose acetate electrophoresis. The combination of sequential degradation and the monitoring of each step by electrophoresis and densitometry permitted the identification and quantitation of all the GAGs on a microscale basis.

Original languageEnglish (US)
Pages (from-to)416-422
Number of pages7
JournalAnalytical Biochemistry
Volume113
Issue number2
DOIs
StatePublished - May 15 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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