Microarray detection of duplex and triplex DNA binders with DNA-modified gold nanoparticles

Abigail K.R. Lytton-Jean, Su Han Min, Chad A. Mirkin*

*Corresponding author for this work

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

We have designed a chip-based assay, using microarray technology, for determining the relative binding affinities of duplex and triplex DNA binders. This assay combines the high discrimination capabilities afforded by DNA-modified Au nanoparticles with the high-throughput capabilities of DNA microarrays. The detection and screening of duplex DNA binders are important because these molecules, in many cases, are potential anticancer agents as well as toxins. Triplex DNA binders are also promising drug candidates. These molecules, in conjunction with triplex-forming oligonucleotides, could potentially be used to achieve control of gene expression by interfering with transcription factors that bind to DNA. Therefore, the ability to screen for these molecules in a high-throughput fashion could dramatically improve the drug screening process. The assay reported here provides excellent discrimination between strong, intermediate, and weak duplex and triplex DNA binders in a high-throughput fashion.

Original languageEnglish (US)
Pages (from-to)6037-6041
Number of pages5
JournalAnalytical Chemistry
Volume79
Issue number15
DOIs
StatePublished - Aug 1 2007

ASJC Scopus subject areas

  • Analytical Chemistry

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