Microbial community and metabolic succession on common building materials under high relative humidity conditions

Simon Lax, Cesar Cardona, Dan Zhao, Valerie J. Winton, Daniel Raba, Gabriel Goodney, Peng Gao, Neil Gottel, Erica Hartmann, Paul Martin Thomas, Scott Kelley, Brent Stephens, Jack A. Gilbert*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To better understand the colonization and succession of microbial communities in the built environment, we employed particle counting, 16S and ITS rRNA amplicon sequencing, and metabolomics profiling to characterize the bacterial and fungal diversity on tiles composed of four commonly used building materials. We varied the inoculating location and the available moisture to assess how these factors influence community structure, and linked community membership to the metabolic profiles of the tiles. We found significantly greater fungal growth and lower taxonomic and metabolite diversity on wet compared to dry tiles. Material played a significant role in shaping the bacterial community and the metabolic profiles, but did not influence the fungal community. Strikingly, inoculation location had a primary effect on the microbial community structure, while moisture was important in driving community succession. This provides evidence of the microbial metabolism dynamics on surface materials influenced by source of colonization and surface material type.

Original languageEnglish (US)
Title of host publication15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018
PublisherInternational Society of Indoor Air Quality and Climate
ISBN (Electronic)9781713826514
StatePublished - 2018
Event15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018 - Philadelphia, United States
Duration: Jul 22 2018Jul 27 2018

Publication series

Name15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018

Conference

Conference15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018
Country/TerritoryUnited States
CityPhiladelphia
Period7/22/187/27/18

Keywords

  • Built environment microbiome
  • Fungal growth
  • Metabolomics
  • Microbial communities
  • Microbial metabolism

ASJC Scopus subject areas

  • Pollution

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