Abstract
A simple technique for microdissection of specific region of human high resolution banded chromosome, followed by polymerase chain reaction (PCR), and microcloning was successfully used to microdissect 4 chromosomal pieces from the distal one third from band 11.2 to the terminal of the short arm of Y chromosome where the testis determining factor is located; 3.6 x 10(4) clones were obtained after 30 cycles of PCR. We analysed 41 clones with insert. The size of insert ranges from 140 to 350 bp (average 250 bp). A Southern blot analysis was done for one of them, and a 2.5 kb Hind III fragment was detected.
Original language | English (US) |
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Pages (from-to) | 653-657 |
Number of pages | 5 |
Journal | Chinese medical journal |
Volume | 104 |
Issue number | 8 |
State | Published - Aug 1991 |
ASJC Scopus subject areas
- General Medicine