Microfluidic gas/liquid toxicity sensing through the chemotaxis of euglena cells confined in a micro-aquarium

K. Ozasa*, J. Lee, S. Song, M. Hara, M. Maeda

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a new biosensor device for environmental gas/liquid toxicity sensing, which utilizes the chemotaxis of microbial cells confined in a micro-aquarium. The device has an isolated micro-aquarium to confine microbial cells separately from flowing gas/liquid in two bypass microchannels, and the molecules of the flowing gas/liquid permeate from the microchannels into the micro-aquarium through porous poly-dimethylsiloxane (PDMS) walls. We demonstrate that CO2-gas or ethanol liquid can be sensed by the device through the chemotaxis of Euglena gracilis. Since Euglena cells in the device can be maintained for more than two weeks, our chemotaxis device is suitable for long-term toxicity monitoring of environmental gases/liquids as well as for microbiological study on chemotactic reactions of motile microbes.

Original languageEnglish (US)
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1420-1422
Number of pages3
ISBN (Print)9780979806452
StatePublished - 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period10/28/1211/1/12

Keywords

  • Chemotaxis
  • Euglena gracilis
  • Gas diffusion
  • Micro-aquarium
  • PDMS
  • Toxicity monitoring

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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