Micromechanical studies of mitotic chromosomes

Research output: Contribution to journalReview article

80 Scopus citations

Abstract

Mitotic chromosomes respond elastically to forces in the nanonewton range, a property important to transduction of stresses used as mechanical regulatory signals during cell division. In addition to being important biologically, chromosome elasticity can be used as a tool for investigating the folding of chromatin. This paper reviews experiments studying stretching and bending stiffness of mitotic chromosomes, plus experiments where changes in chromosome elasticity resulting from chemical and enzyme treatments were used to analyse connectivity of chromatin inside chromosomes. Experiments with nucleases indicate that non-DNA elements constraining mitotic chromatin must be isolated from one another, leading to the conclusion that mitotic chromosomes have a chromatin 'network' or 'gel' organization, with stretches of chromatin strung between 'crosslinking' points. The as-yet unresolved questions of the identities of the putative chromatin crosslinkers and their organization inside mitotic chromosomes are discussed.

Original languageEnglish (US)
Pages (from-to)469-497
Number of pages29
JournalChromosome Research
Volume16
Issue number3
DOIs
StatePublished - May 1 2008

Keywords

  • Chromatin
  • Chromosome
  • Chromosome mechanics
  • Mitosis
  • Single-molecule experiments

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Micromechanical studies of mitotic chromosomes'. Together they form a unique fingerprint.

  • Cite this