Micropatterned surfaces for control of cell shape, position, and function

Christopher S. Chen, Milan Mrksich, Sui Huang, Goerge M. Whitesides, Donald E. Ingber*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

683 Scopus citations


The control of cell position and function is a fundamental focus in the development of applications ranging from cellular biosensors to tissue engineering. Using microcontact printing of self-assembled monolayers (SAMs) of alkanethiolates on gold, we manufactured substrates that contained micrometer-scale islands of extracellular matrix (ECM) separated by nonadhesive regions such that the pattern of islands determined the distribution and position of bovine and human endothelial cells. In addition, the size and geometry of the islands were shown to control cell shape. Traditional approaches to modulate cell shape, either by attaching suspended cells to microbeads of different sizes or by plating cells on substrates coated with different densities of ECM, suggested that cell shape may play an important role in control of apoptosis as well as growth. Data are presented which show how micropatterned substrates were used to definitively test this hypothesis. Progressively restricting bovine and human endothelial cell extension by culturing cells on smaller and smaller micropatterned adhesive islands regulated a transition from growth to apoptosis on a single continuum of cell spreading, thus confirming the central role of cell shape in cell function. The micropatterning technology is therefore essential not only for construction of biosurface devices but also for the investigation of the fundamental biology of cell-ECM interactions.

Original languageEnglish (US)
Pages (from-to)356-363
Number of pages8
JournalBiotechnology Progress
Issue number3
StatePublished - May 1998

ASJC Scopus subject areas

  • Biotechnology


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