Micropropagation of the rare lakeside daisy (Hymenoxys acaulis var. glabra)

James R. Ault

doi:10.21273/hortsci.37.1.200

Micropropagation of the rare lakeside daisy (Hymenoxys acaulis var. glabra)

James R. Ault^*

^*Corresponding author for this work

Program in Biological Sciences

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Shoot tip and stem segment explants collected from greenhouse-maintained plants of Hymenoxys acaulis var. glabra were cultured in vitro for shoot initiation on a Murashige and Skoog (MS) medium supplemented with 30 g·L^-1 sucrose, 2.5 μM BA, and 7 g·L^-1 agar at a pH of 5.7. Unbranched shoot explants were subcultured to MS medium with 0.0, 0.5, 1, 2, 4 or 8 μM BA for shoot proliferation. A maximum of 10.3 shoots per explant was produced on the medium with 2.0 μM BA. Nonrooted shoots were subcultured to MS medium with 0.0, 0.5, 2, or 8 μM K-IBA for rooting. Maximum rooting was 90% on MS medium with 0.5 μM K-IBA. Rooted shoots were greenhouse-acclimatized for 10 days. Overall survival was 75%. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

Original language	English (US)
Pages (from-to)	200-201
Number of pages	2
Journal	HortScience
Volume	37
Issue number	1
DOIs	https://doi.org/10.21273/hortsci.37.1.200
State	Published - 2002

Keywords

Asteraceae
Auxin
Cytokinin
Federally threatened
Shoot production
Tissue culture

ASJC Scopus subject areas

Horticulture

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title = "Micropropagation of the rare lakeside daisy (Hymenoxys acaulis var. glabra)",

abstract = "Shoot tip and stem segment explants collected from greenhouse-maintained plants of Hymenoxys acaulis var. glabra were cultured in vitro for shoot initiation on a Murashige and Skoog (MS) medium supplemented with 30 g·L-1 sucrose, 2.5 μM BA, and 7 g·L-1 agar at a pH of 5.7. Unbranched shoot explants were subcultured to MS medium with 0.0, 0.5, 1, 2, 4 or 8 μM BA for shoot proliferation. A maximum of 10.3 shoots per explant was produced on the medium with 2.0 μM BA. Nonrooted shoots were subcultured to MS medium with 0.0, 0.5, 2, or 8 μM K-IBA for rooting. Maximum rooting was 90% on MS medium with 0.5 μM K-IBA. Rooted shoots were greenhouse-acclimatized for 10 days. Overall survival was 75%. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).",

keywords = "Asteraceae, Auxin, Cytokinin, Federally threatened, Shoot production, Tissue culture",

author = "Ault, {James R.}",

year = "2002",

doi = "10.21273/hortsci.37.1.200",

language = "English (US)",

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N2 - Shoot tip and stem segment explants collected from greenhouse-maintained plants of Hymenoxys acaulis var. glabra were cultured in vitro for shoot initiation on a Murashige and Skoog (MS) medium supplemented with 30 g·L-1 sucrose, 2.5 μM BA, and 7 g·L-1 agar at a pH of 5.7. Unbranched shoot explants were subcultured to MS medium with 0.0, 0.5, 1, 2, 4 or 8 μM BA for shoot proliferation. A maximum of 10.3 shoots per explant was produced on the medium with 2.0 μM BA. Nonrooted shoots were subcultured to MS medium with 0.0, 0.5, 2, or 8 μM K-IBA for rooting. Maximum rooting was 90% on MS medium with 0.5 μM K-IBA. Rooted shoots were greenhouse-acclimatized for 10 days. Overall survival was 75%. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

AB - Shoot tip and stem segment explants collected from greenhouse-maintained plants of Hymenoxys acaulis var. glabra were cultured in vitro for shoot initiation on a Murashige and Skoog (MS) medium supplemented with 30 g·L-1 sucrose, 2.5 μM BA, and 7 g·L-1 agar at a pH of 5.7. Unbranched shoot explants were subcultured to MS medium with 0.0, 0.5, 1, 2, 4 or 8 μM BA for shoot proliferation. A maximum of 10.3 shoots per explant was produced on the medium with 2.0 μM BA. Nonrooted shoots were subcultured to MS medium with 0.0, 0.5, 2, or 8 μM K-IBA for rooting. Maximum rooting was 90% on MS medium with 0.5 μM K-IBA. Rooted shoots were greenhouse-acclimatized for 10 days. Overall survival was 75%. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

KW - Asteraceae

KW - Auxin

KW - Cytokinin

KW - Federally threatened

KW - Shoot production

KW - Tissue culture

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Micropropagation of the rare lakeside daisy (Hymenoxys acaulis var. glabra)

Abstract

Keywords

ASJC Scopus subject areas

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