Micropropagation of the rare lakeside daisy (Hymenoxys acaulis var. glabra)

James R. Ault*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Shoot tip and stem segment explants collected from greenhouse-maintained plants of Hymenoxys acaulis var. glabra were cultured in vitro for shoot initiation on a Murashige and Skoog (MS) medium supplemented with 30 g·L-1 sucrose, 2.5 μM BA, and 7 g·L-1 agar at a pH of 5.7. Unbranched shoot explants were subcultured to MS medium with 0.0, 0.5, 1, 2, 4 or 8 μM BA for shoot proliferation. A maximum of 10.3 shoots per explant was produced on the medium with 2.0 μM BA. Nonrooted shoots were subcultured to MS medium with 0.0, 0.5, 2, or 8 μM K-IBA for rooting. Maximum rooting was 90% on MS medium with 0.5 μM K-IBA. Rooted shoots were greenhouse-acclimatized for 10 days. Overall survival was 75%. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

Original languageEnglish (US)
Pages (from-to)200-201
Number of pages2
JournalHortScience
Volume37
Issue number1
DOIs
StatePublished - 2002

Keywords

  • Asteraceae
  • Auxin
  • Cytokinin
  • Federally threatened
  • Shoot production
  • Tissue culture

ASJC Scopus subject areas

  • Horticulture

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