MicroRNA-103/107 family regulates multiple epithelial stem cell characteristics

Han Peng, Jong Kook Park, Julia Katsnelson, Nihal Kaplan, Wending Yang, Spiro Getsios, Robert M. Lavker*

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The stem cell niche is thought to affect cell cycle quiescence, proliferative capacity, and communication between stem cells and their neighbors. How these activities are controlled is not completely understood. Here we define a microRNA family (miRs-103/107) preferentially expressed in the stem cell-enriched limbal epithelium that regulates and integrates these stem cell characteristics. miRs-103/107 target the ribosomal kinase p90RSK2, thereby arresting cells in G0/G1 and contributing to a slow-cycling phenotype. Furthermore, miRs-103/107 increase the proliferative capacity of keratinocytes by targeting Wnt3a, which enhances Sox9 and YAP1 levels and thus promotes a stem cell phenotype. This miRNA family also regulates keratinocyte cell-cell communication by targeting: (a) the scaffolding protein NEDD9, preserving E-cadherin-mediated cell adhesion; and (b) the tyrosine phosphatase PTPRM, which negatively regulates connexin 43-based gap junctions. We propose that such regulation of cell communication and adhesion molecules maintains the integrity of the stem cell niche ultimately preserving self-renewal, a hallmark of epithelial stem cells. Stem Cells 2015;33:1642-1656

Original languageEnglish (US)
Pages (from-to)1642-1656
Number of pages15
JournalStem Cells
Volume33
Issue number5
DOIs
StatePublished - May 1 2015

Keywords

  • Adherens junctions
  • Cell cycle
  • Cell-cell communication
  • Gap junctions
  • Proliferative capacity
  • Wnt signaling

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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