MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion

Jessica Bockhorn; Kathy Yee; Ya Fang Chang; Aleix Prat; Dezheng Huo; Chika Nwachukwu; Rachel Dalton; Simo Huang; Kaitlin E. Swanson; Charles M. Perou; Olufunmilayo I. Olopade; Michael F. Clarke; Geoffrey L. Greene; Huiping Liu

doi:10.1007/s10549-012-2346-4

MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion

Jessica Bockhorn, Kathy Yee, Ya Fang Chang, Aleix Prat, Dezheng Huo, Chika Nwachukwu, Rachel Dalton, Simo Huang, Kaitlin E. Swanson, Charles M. Perou, Olufunmilayo I. Olopade, Michael F. Clarke, Geoffrey L. Greene^*, Huiping Liu

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

Metastasis remains a significant challenge in treating cancer. A better understanding of the molecular mechanisms underlying metastasis is needed to develop more effective treatments. Here, we show that human breast tumor biomarker miR-30c regulates invasion by targeting the cytoskeleton network genes encoding twinfilin 1 (TWF1) and vimentin (VIM). Both VIM and TWF1 have been shown to regulate epithelial-to-mesenchymal transition. Similar to TWF1, VIM also regulates F-actin formation, a key component of cellular transition to a more invasive mesenchymal phenotype. To further characterize the role of the TWF1 pathway in breast cancer, we found that IL-11 is an important target of TWF1 that regulates breast cancer cell invasion and STAT3 phosphorylation. The miR-30c-VIM/TWF1 signaling cascade is also associated with clinical outcome in breast cancer patients.

Original language	English (US)
Pages (from-to)	373-382
Number of pages	10
Journal	Breast Cancer Research and Treatment
Volume	137
Issue number	2
DOIs	https://doi.org/10.1007/s10549-012-2346-4
State	Published - Jan 2013

Funding

Funding This study was supported in part by The University of Chicago Women’s Board (J.B.), National Institutes of Health (NIH) T90 Regenerative Medicine Training Program DK070103-05, Department of Defense Breast Cancer Research Program W81XWH-09-1-0331, Paul Calabresi K12 Award 1K12CA139160-02, NCI K99 CA160638-01A1, Chicago Fellows Program at the University of Chicago, and the University of Chicago Clinical and Translational Science Award (UL1 RR024999) (H.L.), funds from the Sociedad Española de Oncología Médica (SEOM) (A.P.), the Breast SPORE at University of North Carolina 5-P50-CA58223-17 (A.P. and C.M.P.), the Breast SPORE at the University of Chicago P50CA125183-05, the Doris Duke Charitable Foundation (O.I.O and C.N.), The University of Chicago Cancer Research Center Pilot Fund, BSD Imaging Research Institute Pilot Research Projects Using Animal Imaging, UCMC/Northshore Collaborative Funds, a Carole and Gordon Segal Grant (G.L.G.), and the Virginia and D. K. Ludwig Fund (G.L.G. and H.L.), NIH Grants U54 CA 126524 and P01 CA139490 (M.F.C.), the Breast Cancer Research Foundation (M.F.C., O.I.O. and C.M.P), and the University of Chicago Cancer Center Support Grant CA 014599.

Keywords

Breast tumor invasion
IL-11
TWF1
VIM
miR-30c

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{46a7966e9b1d4017afc69855289c376f,

title = "MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion",

abstract = "Metastasis remains a significant challenge in treating cancer. A better understanding of the molecular mechanisms underlying metastasis is needed to develop more effective treatments. Here, we show that human breast tumor biomarker miR-30c regulates invasion by targeting the cytoskeleton network genes encoding twinfilin 1 (TWF1) and vimentin (VIM). Both VIM and TWF1 have been shown to regulate epithelial-to-mesenchymal transition. Similar to TWF1, VIM also regulates F-actin formation, a key component of cellular transition to a more invasive mesenchymal phenotype. To further characterize the role of the TWF1 pathway in breast cancer, we found that IL-11 is an important target of TWF1 that regulates breast cancer cell invasion and STAT3 phosphorylation. The miR-30c-VIM/TWF1 signaling cascade is also associated with clinical outcome in breast cancer patients.",

keywords = "Breast tumor invasion, IL-11, TWF1, VIM, miR-30c",

author = "Jessica Bockhorn and Kathy Yee and Chang, {Ya Fang} and Aleix Prat and Dezheng Huo and Chika Nwachukwu and Rachel Dalton and Simo Huang and Swanson, {Kaitlin E.} and Perou, {Charles M.} and Olopade, {Olufunmilayo I.} and Clarke, {Michael F.} and Greene, {Geoffrey L.} and Huiping Liu",

note = "Funding Information: Funding This study was supported in part by The University of Chicago Women{\textquoteright}s Board (J.B.), National Institutes of Health (NIH) T90 Regenerative Medicine Training Program DK070103-05, Department of Defense Breast Cancer Research Program W81XWH-09-1-0331, Paul Calabresi K12 Award 1K12CA139160-02, NCI K99 CA160638-01A1, Chicago Fellows Program at the University of Chicago, and the University of Chicago Clinical and Translational Science Award (UL1 RR024999) (H.L.), funds from the Sociedad Espa{\~n}ola de Oncolog{\'i}a M{\'e}dica (SEOM) (A.P.), the Breast SPORE at University of North Carolina 5-P50-CA58223-17 (A.P. and C.M.P.), the Breast SPORE at the University of Chicago P50CA125183-05, the Doris Duke Charitable Foundation (O.I.O and C.N.), The University of Chicago Cancer Research Center Pilot Fund, BSD Imaging Research Institute Pilot Research Projects Using Animal Imaging, UCMC/Northshore Collaborative Funds, a Carole and Gordon Segal Grant (G.L.G.), and the Virginia and D. K. Ludwig Fund (G.L.G. and H.L.), NIH Grants U54 CA 126524 and P01 CA139490 (M.F.C.), the Breast Cancer Research Foundation (M.F.C., O.I.O. and C.M.P), and the University of Chicago Cancer Center Support Grant CA 014599.",

year = "2013",

month = jan,

doi = "10.1007/s10549-012-2346-4",

language = "English (US)",

volume = "137",

pages = "373--382",

journal = "Breast Cancer Research and Treatment",

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T1 - MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion

AU - Bockhorn, Jessica

AU - Yee, Kathy

AU - Chang, Ya Fang

AU - Prat, Aleix

AU - Huo, Dezheng

AU - Nwachukwu, Chika

AU - Dalton, Rachel

AU - Huang, Simo

AU - Swanson, Kaitlin E.

AU - Perou, Charles M.

AU - Olopade, Olufunmilayo I.

AU - Clarke, Michael F.

AU - Greene, Geoffrey L.

AU - Liu, Huiping

N1 - Funding Information: Funding This study was supported in part by The University of Chicago Women’s Board (J.B.), National Institutes of Health (NIH) T90 Regenerative Medicine Training Program DK070103-05, Department of Defense Breast Cancer Research Program W81XWH-09-1-0331, Paul Calabresi K12 Award 1K12CA139160-02, NCI K99 CA160638-01A1, Chicago Fellows Program at the University of Chicago, and the University of Chicago Clinical and Translational Science Award (UL1 RR024999) (H.L.), funds from the Sociedad Española de Oncología Médica (SEOM) (A.P.), the Breast SPORE at University of North Carolina 5-P50-CA58223-17 (A.P. and C.M.P.), the Breast SPORE at the University of Chicago P50CA125183-05, the Doris Duke Charitable Foundation (O.I.O and C.N.), The University of Chicago Cancer Research Center Pilot Fund, BSD Imaging Research Institute Pilot Research Projects Using Animal Imaging, UCMC/Northshore Collaborative Funds, a Carole and Gordon Segal Grant (G.L.G.), and the Virginia and D. K. Ludwig Fund (G.L.G. and H.L.), NIH Grants U54 CA 126524 and P01 CA139490 (M.F.C.), the Breast Cancer Research Foundation (M.F.C., O.I.O. and C.M.P), and the University of Chicago Cancer Center Support Grant CA 014599.

PY - 2013/1

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N2 - Metastasis remains a significant challenge in treating cancer. A better understanding of the molecular mechanisms underlying metastasis is needed to develop more effective treatments. Here, we show that human breast tumor biomarker miR-30c regulates invasion by targeting the cytoskeleton network genes encoding twinfilin 1 (TWF1) and vimentin (VIM). Both VIM and TWF1 have been shown to regulate epithelial-to-mesenchymal transition. Similar to TWF1, VIM also regulates F-actin formation, a key component of cellular transition to a more invasive mesenchymal phenotype. To further characterize the role of the TWF1 pathway in breast cancer, we found that IL-11 is an important target of TWF1 that regulates breast cancer cell invasion and STAT3 phosphorylation. The miR-30c-VIM/TWF1 signaling cascade is also associated with clinical outcome in breast cancer patients.

AB - Metastasis remains a significant challenge in treating cancer. A better understanding of the molecular mechanisms underlying metastasis is needed to develop more effective treatments. Here, we show that human breast tumor biomarker miR-30c regulates invasion by targeting the cytoskeleton network genes encoding twinfilin 1 (TWF1) and vimentin (VIM). Both VIM and TWF1 have been shown to regulate epithelial-to-mesenchymal transition. Similar to TWF1, VIM also regulates F-actin formation, a key component of cellular transition to a more invasive mesenchymal phenotype. To further characterize the role of the TWF1 pathway in breast cancer, we found that IL-11 is an important target of TWF1 that regulates breast cancer cell invasion and STAT3 phosphorylation. The miR-30c-VIM/TWF1 signaling cascade is also associated with clinical outcome in breast cancer patients.

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KW - TWF1

KW - VIM

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SN - 0167-6806

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JO - Breast Cancer Research and Treatment

JF - Breast Cancer Research and Treatment

IS - 2

ER -

MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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